The product of this gene functions in the nucleotideexcision repair pathway, and is required for the repair of DNAlesions such as those induced by UV light or formed byelectrophilic compounds including cisplatin. The encoded proteinforms a heterodimer with the XPF endonuclease (also known asERCC4), and the heterodimeric endonuclease catalyzes the 5"incision in the process of excising the DNA lesion. Theheterodimeric endonuclease is also involved in recombinational DNArepair and in the repair of inter-strand crosslinks. Mutations inthis gene result in cerebrooculofacioskeletal syndrome, andpolymorphisms that alter expression of this gene may play a role incarcinogenesis. Multiple transcript variants encoding differentisoforms have been found for this gene. The last exon of this geneoverlaps with the CD3e molecule, epsilon associated protein gene onthe opposite strand.
Titration of the ERCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 268-297 from the human protein was used as the immunogen for this ERCC1 antibody.
Aliquot the ERCC1 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.
