Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. It belongs to the CXC chemokine family. The gene contains 3 exons spanning approximately 1 kb. It is encoded on a 10-kb EcoRI fragment of genomic DNA. By in situ hybridization, the gene is mapped to chromosome 4q12-q21. CXCL4s major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, the cytokine probably has a role in inflammation and wound repair.
The stated application concentrations are suggested starting points.Titration of the CXCL4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Amino acids 85-101 (DLQAPLYKKIIKKLLES-human) were used as the immunogen for this CXCL4 antibody.
After reconstitution, the CXCL4 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.