Exalpha蚂蚁淘在线

ThereactivityoftheantiserumisdirectedtothesubclassIgG2a.ItreactsstronglywithIg2aoftheallelictypesIgh-1aandIgh-1b,whichincludeBALB/C,C57BL,CBA/J,SJ/JandSM/J.WhenusedfrotheidentificationofIgG2ainothermousestrainsthereactionmaybeweaker.Theantiserumcontainsantibodiestoisoandallospecificdeterminants.ItdoesnotreactwithothersubclassesofIgG,IgG/Fabfragments,IgMandIgAoranynon-Igproteininmouseserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.InimmunocytochemicalandimmunohistochemicalsingleanddoublestainingforthedetectionofIgG2aatthecellularandsubcellularlevelbystainingofappropriatelytreatedcellandtissuesubstrates;asindicatingsecondantibodyconjugatetoanalyzeorseparatehumancellsafterreactingwithmonoclonalmouseantibodiesinimmunofluorescencemicroscopy,fluorescenceactivatedcellsortingandflowcytometry;toidentifyandmeasureIgG2ainmouseserumorotherbodyfluids.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.Workingdilutionsareusuallybetween1:10and1:40,dependingonthemethodused.

Host

Goat

SpeciesReactivity

Mouse

ProductUsage

Tetramethylrhodamineisothiocyanate-conjugatedIgGfractionofpolyclonalGoatantiSerumtoMouseIgG2a,subclassspecific

ProductType

SecondaryAntibodies

Applications

ELISA,Immunocytochemistry,Immunohistochemistry(frozen),(In)directimmunofluorescence

FieldsofInterest

Veterinary

ProductSource

PoolsofpurifiedhomogenousIgG2aisolatedfrommouseserumofstrainsbelongingtotheallelictypesIgh-1aandIgh-1b.Freund

ProductFormulation

TRITC-coupledpurifiedhyperimmunegoatIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded,asitmayinterferewiththeantibodyactivity.

ProductSpecificity

Thisimmunoconjugateisnotspecies-specificsinceinter-speciescross-reactivityisanormalfeatureofantiseratoimmunoglobulins.Howeverthisconjugatehasbeenpassedoverappropriateimmuno-adsorbentstoremoveantibodiescross-reactingwithHumanimmunoglobulins.ThisrendersitspecificforuseintestsystemscontainingmaterialofHumanorigin(e.g.Humantissue/MousemonoclonalantibodytoaHumantissueconstituent/antiMouseIgisotype-specificimmunoconjugate.

StorageConditions

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4

Exalpha血清是凝结后残留的全血液体部分。通过离心将该液体部分与凝结的细胞和凝血因子分离。它包含蛋白质的混合物，包括抗体（免疫球蛋白），血清白蛋白，α和β球蛋白以及调节蛋白，例如激素和酶。我们从人类，灵长类和兽医物种中提取的血清和牛奶可用于多种用途；研究血清或牛奶的正常蛋白质组成，作为参考蛋白质，阻断剂或阴性对照。

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