Exalpha蚂蚁淘在线

ThereactivityoftheantiserumisdirectedtotheFcandFabsubunitsoftheIgGmolecule.ItincludesacertaindegreeofreactivitywithotherimmunoglobulinsviathecommonFabportion.Itdoesnotreactwithanynon-Igproteininmouseserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.ToidentifyandmeasureIgG,antigenorantibody,atthecellularandsubcellularlevelbyimmunofluorescencestainingofappropriatelytreatedcellandtissuesubstrates,andtodemonstratecirculatingantibodiesinSEROdiagnosticmicroBIOLOGyandautoimmunediseases;toidentifyaspecificantigenorimmunecomplexusingareferenceantibodyofmouseorigininthemiddlelayeroftheindirecttestprocedure.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.Workingdilutionsareusuallybetween1:20and1:80.

Host

Goat

SpeciesReactivity

Mouse

ProductUsage

Fluoresceinisothiocyanate-conjugatedIgGfractionofpolyclonalGoatantiSerumtoMouseIgG,heavyandlightchains

ProductType

SecondaryAntibodies

Applications

ELISA,Immunocytochemistry,Immunohistochemistry(frozen),(In)directimmunofluorescence

FieldsofInterest

Veterinary

ProductSource

PurifiednormalIgGincludingallknownsubclassesisolatedfrompooledmouseserum.Freund

ProductFormulation

FITC-coupledpurifiedhyperimmunegoatIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded.

ProductSpecificity

Inter-speciescross-reactivityisanormalfeatureofantibodiestoimmunoglobulins,sinceIgofdifferentspeciesfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiSerumhasnotbeentestedindetail.

StorageConditions

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4

Exalpha血清是凝结后残留的全血液体部分。通过离心将该液体部分与凝结的细胞和凝血因子分离。它包含蛋白质的混合物，包括抗体（免疫球蛋白），血清白蛋白，α和β球蛋白以及调节蛋白，例如激素和酶。我们从人类，灵长类和兽医物种中提取的血清和牛奶可用于多种用途；研究血清或牛奶的正常蛋白质组成，作为参考蛋白质，阻断剂或阴性对照。

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