Exalpha蚂蚁淘在线

ThereactivityoftheantiserumisdirectedtotheFcsubunitoftheIgGmoleculewhichexpressesstrictisotypic(class)specificity.Itdoesnotreactwithanynon-Igproteininhumanserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.ThisantiserumisintendedtodetectandidentifyIgGinserumorotherbodyfluidsusingtheimmunofixationtechnique.Likeimmunoelectrophoresisimmunofixationisessentiallyatwosteptechnique.Proteinsinacomplexmixtureareseparatedbyelectrophoresisinagelcarrier,followedbyimmunoprecipitationinsituwiththeantiserum.Non-precipitatedproteinsareremovedbywashingandtheprecipitatedcomplexisrevealedwithaproteinstainwhichallowsitsexactlocalization.Immunofixationmaybethemethodofchoicewheneverahighlevelofsensitivityisrequiredtoidentifyaminorproteincomponentagainstahighbackgroundofotherproteins.Itenablesthedetectionandidentificationofmorethanoneparaproteinsinserumoroffreelightchain.Thedetectionlimitisapproximately0.5to1mg/mlinthepresenceofnormallevelsofimmunoglobulins.Thisproductisnotpre-diluted.Theoptimumworkingdilutionofeachproductshouldbeestablishedbytitrationbeforebeingused.

Host

Goat

SpeciesReactivity

Human

ProductUsage

IgfractionofpolyclonalGoatantiSerumtoHumanimmunoglobulinIgG,Fcspecific

ProductType

SecondaryAntibodies

Applications

Immunoprecipitation

FieldsofInterest

Immunology

ProductSource

HighlypurifiedpolyclonalIgGisolatedfromhumanserum.Freund

ProductFormulation

GoatIglyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded,asitmayinterferewiththeantibodyactivity.

ProductSpecificity

Inter-speciescross-reactivityisanormalfeatureofantibodiestomammalianimmunoglobulins,sincehomologousproteinsofdifferentspeciesfrequentlyshareantigenicdeterminants.ThedegreeofcrossreactivityisalsodependentontheconcentRationsofthereactantsandthesensitivityoftheassayarrangement.ThisantiSerumfractionhasnotbeentestedforcross-reactivity.

StorageConditions

Thelyophilizedproductisshippedatambienttemperatureandmaybestoredat+4

Exalpha血清是凝结后残留的全血液体部分。通过离心将该液体部分与凝结的细胞和凝血因子分离。它包含蛋白质的混合物，包括抗体（免疫球蛋白），血清白蛋白，α和β球蛋白以及调节蛋白，例如激素和酶。我们从人类，灵长类和兽医物种中提取的血清和牛奶可用于多种用途；研究血清或牛奶的正常蛋白质组成，作为参考蛋白质，阻断剂或阴性对照。

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