| Isotype | IgG |
| Product Type | Polyclonal Antibody |
| Units | 100 µg |
| Host | Sheep |
| Species reactivity | HumanRat |
| Application | Immunoprecipitation |
BackgroundThe immunogen for the anti-EIA was a recombinant E1Aproteinrecognizinga43kD protein.EIAgeneencodespotentonco-proteinsthatmodifythenormaltranscriptionalgrowth regulation of key cellular genes and thus alter cell cycle control. E1A protein has both transforming and trans-activating activities. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Disrupts the function of host retinoblastoma protein RB1/pRb and isoform early E1A 26 kDa protein stabilizes TP53, which are key regulators of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif
Synonyms: E1A Early Adenovirus region 1
Source
Immunogen: Recombinant E1A protein recognizinga43kD protein
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
ApplicationsWestern Blot suggested dilution1- 10 ug /ml
Functional Analysis: Western Blotting
Positive Control: 293 cells are human embryonic kidney cells that have been transformed with the adenovirus genes E1A and E1B, which are required for adenovirus propagation. This cell line can be used as a positive control for this antibody. This antibody is suitable for use with his tagged or gst tagged recombinant proteins.
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: room temperature
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
References1. Chatton, B., et al. "Transcriptional activation by the adenovirus larger E1a product is mediated by members of the cellular transcription factor ATF family which can directly associate with E1a." Mol. Cell. Biol., 13, 561-570 (1993)
Protein Reference(s)
Database Name: UniProt
Accession number: P03254 (Human)
Species Accession: Human
Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
