| Product Type | Polyclonal Antibody |
| Units | 100 µg |
| Host | Rabbit |
| Species reactivity | Human |
| Application | Western BlotWestern Blotting |
BackgroundSphingomyelin phosphodiesterase 3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine.Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these two cellular events independently. It also hydrolyzes sphingosylphosphocholine. Sphingomyelin phosphodiesterase 3 regulates the cell cycle by acting as a growth suppressor in confluent cells. It likely acts as a regulator of postnatal development and participates in bone and dentin mineralization.It is activated by unsaturated fatty acids and phosphatidylserine.it is predominantly expressed in the brain.
Synonyms: nSMase2; Neutral sphingomyelinase 2; EC 3.1.4.12
Source
Immunogen: Synthetic peptide derived from human sphingomyelin phosphodiesterase 3
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
ApplicationsAntibody can be used for Western blotting (1-5 µg/ml starting dilution).Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Predominantly expressed in the brain.
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
References1. Hofmann, K., et al. Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase.; Proc. Natl. Acad. Sci. U.S.A. 97:5895-5900(2000).2. Miura, Y., et al. Hydrolysis of sphingosylphosphocholine by neutral sphingomyelinases.; FEBS Lett. 557:288-292(2004).3. Marchesini, N., et al. Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells.; J. Biol. Chem. 279:25101-25111(2004).
Protein Reference(s)
Database Name: UniProt
Accession number: Q53YV1 (Human)
Species Accession: Human
Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
