| Clone | Polyclonal |
| Units | 1ml |
| Host | Rabbit |
BackgroundThedefinedantibodyspecificityisdirectedtoalbuminastestedagainstbovinesera.InimmunoelectrophoresisanddoublerADIalimmunodiffusion(Ouchterlony),usingvariousantiserumconcentrationsagainstappropriateconcentrationsoftheimmunogen,asinglecharacteristicprecipitinlineisobtainedwhichshowsareactionofidentitywiththeprecipitinlinesobtainedagainstbovineserumandthepurifiedalbumin.Asreagentforthedirectdetectionofalbumininbovinecells,tissuesandbodyfluidsinimmunofluorescencetechniques.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.
SourceAlbuminisastablesmallpolypeptidewithastrongantigenicity.Itsmolecularweightisabout69,000.Ithasahighmobilityinelectrophoresis,showsmacro-heterogeneityespeciallyunderpathologicalconditionsanditcanbindalargenumberofphysiologicalandnon-physiologicalmolecules.Albuminisisolatedfrombovineserumbysequentialprecipitationandpurifiedbyionexchangechromatographyandaffinitychromatography.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.
ProductPurifiedhyperimmunerabbitIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded,asitmayinterferewiththeantibodyactivity.Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.
ProductForm:FITC
Formulation:PurifiedhyperimmunerabbitIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded,asitmayinterferewiththeantibodyactivity.
SpecificityFluoresceinisothiocyanate-conjugatedIgGfractionofpolyclonalRabbitantiSerumtoBovinealbumin.
SpeciesReactivity:Inter-speciescross-reactivityisanormalfeatureofantibodiestomammalianSerumproteins,sincehomologousproteinsofdifferentspeciesfrequentlyshareantigenicdeterminants.ThedegreeofcrossreactivityisalsodependentontheconcentRationsofthereactantsandthesensitivityoftheassayarrangement.ThisantiSerumhasbeentestedindoubleradialimmunodiffusionwiththefollowingresults:Cat-Chicken-Dog+Duck-Goat++GuineaPig++Hamster+Horse+Human+Monkey+Mouse++pigeon-Rabbit-Rat±Sheep-Swine+Anegativecross-reactionindoubleradialimmunodiffusiondoesnotexcludesomereactioninmoresensitivetechniques.
ApplicationsELISA,Immunocytochemistry,Immunohistochemistry(frozen),(In)directimmunofluorescence.
StorageThelyophilizedproductisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyinthedarkatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheproduct.
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butExalphaBIOLOGicalsacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.
Exalpha血清是凝结后残留的全血液体部分。通过离心将该液体部分与凝结的细胞和凝血因子分离。它包含蛋白质的混合物，包括抗体（免疫球蛋白），血清白蛋白，α和β球蛋白以及调节蛋白，例如激素和酶。我们从人类，灵长类和兽医物种中提取的血清和牛奶可用于多种用途；研究血清或牛奶的正常蛋白质组成，作为参考蛋白质，阻断剂或阴性对照。
