| Clone | TE111.5D11 |
| Isotype | IgG1 |
| Product Type | Monoclonal Antibody |
| Units | 100 µg |
| Host | Mouse |
| Species reactivity | ChickenCowDogHamsterHumanMousePigRabbitRatSheep |
| Application | Binding blockingChromatin PrecipitationFunctional InhibitionGel SupershiftImmunoprecipitationWestern Blotting |
BackgroundThe estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE)constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland.Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Source
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with the 35 kDa C-terminal fragment (aa 302-595) of human ER expressed in E. coli and mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
ApplicationsThis antibody can be used gel supershift assays (neat concentration), immunoprecipitation on native and denatured lysate (2 µg/mg of protein lysate), Western blotting (1-2 µg/ml) and chromatin precipitation.Can also be used to block binding of estrogen to the estrogen receptor.Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: T47D Cells
StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: Products are stable for one year from purchase when stored properly
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
References1. Abbondanza, C. et. al. "Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor." Steroids, 1993, 58, 4-12
Protein Reference(s)
Database Name: SwissProt
Accession number: P11474
Species Accession: Human
Safety Datasheet(s) for this product:EA_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium AzideV2.pdf
