OVERVIEW:

Synonyms	Monoclonal AKT1 antibody, Anti-AKT1 antibody, AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, AKT1, AKT 1, AKT-1, AKT 1 antibody, AKT-1 antibody
Cross Reactivity	Human, Dog, Rat, Monkey, Mouse
Applications	FC, IF, IHC, WB
Immunogen	AKT1 antibody was raised in Mouse using full length human recombinant protein of human AKT1 produced in HEK293T cell as the immunogen.

SPECIFICATIONS:

Host	Mouse
Clone	4E10
Isotype	IgG1
Method of Purification	AKT1 antibody was purified by affinity chromatography.
Form & Buffer	Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration	0.5-1 mg/ml

STORAGE & SAFETY:

Storage	Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Usage Assay Information:

Usage Recommendations	IF: 1:100, FC: 1:100, IHC: 1:150, WB: 1:500-2000

GENERAL INFORMATION:

Biological Significance

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival.

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