Type
Recombinant
Description
Total551AA.MW:62.5kDa(calculated).UniProtKBacc.no.P07196(Ser2-Asp543).N-terminalHis-tag(9extraAA).ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
MKHHHHHHASSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis:>90%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanNF-L(E.coli):
- M.W.Marker–14,21,31,45,66,97kDa
- Reducedandboiledsample,2.5μg/lane
- Non-reducedandnon-boiledsample,2.5μg/lane
Endotoxin
<1.0 EU/µg
Formulation
Filtered(0.4μm)andlyophilizedfrom0.5mg/mlsolutionin15mMTris,85mMGlycine,pH8.5.
Reconstitution
Add200µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.
