Type

Recombinant

Description

Total466AA.MW:53.0kDa(calculated).UniProtKBacc.No.P08254(Tyr18–Cys477).C-terminalHis-tag(6AA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

YPLDGAARGEDTSMNLVQKYLENYYDLEKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNCHHHHHH

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis:>95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanMatrixMetalloproteinase-3:

1. M.W.Marker–14,21,31,45,66,97kDa
2. Reducedandboiledsample,2.5μg/lane
3. Non-reducedandnon-boiledsample,2.5μg/lane

Endotoxin

<1.0 EU/µg

Formulation

Filtered(0.4μm)andlyophilizedfrom0.5mg/mlsolutioninphosphatebufferedsalinepH8.0+5%(w/v)Threalose.

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.