Type

Recombinant

Description

Macrophagemigrationinhibitoryfactor(MIF)isasmallsecretedproteinthatcanactasapleiotropicpro-inflammatorycytokine,aswellasanenzyme.MIFpro-inflammatoryactivitycanbeinitiatedbysignalingthroughCD74andCD44,resultinginthesecretionofTNF-a,IL-1,IL-6,IL-8,andvariousMMPs.TheenzymaticactivityofMIFischaracterizedbyitsABIlitytoactasatautomerase,capableofcatalyzingtheketo-to-enolisomerizationofketo-phenylpyruvateandL-dopachrome.ItappearsasthoughMIFcatalyticactivityisdependentuponatrimericconfigurationandafreeN-terminalprolineresidue.Insectcell-derivedRecombinantHumanMIFisa15 kDaproteincontaining124 aminoacidresidues,includinganN-terminalHis-tag.

AminoAcidSequence

HHHHHHHHAMPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA

Source

Hi-5Insectcells

Purity

98%

BIOLOGicalActivity

Determinedbyitsabilitytoinhibitmonocytemigration.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C