Type
Native
Description
Nativeproteinisolatedfrompooledhumanserum,312AA.MW:34,35kDa(calculatedwithoutglycosylation).ProteinidentityconfirmedbyLC-MS/MS(NCBIno.gi|16418467).
AminoAcidSequence
VTLSPKDCQVFRSDHGSSISCQPPAEIPGYLPADTVHLAVEFFNLTHLPANLLQGASKLQELHLSSNGLESLSPEFLRPVPQLRVLDLTRNALTGLPPGLFQASATLDTLVLKENQLEVLEVSWLHGLKALGHLDLSGNRLRKLPPGLLANFTLLRTLDLGENQLETLPPDLLRGPLQLERLHLEGNKLQVLGKDLLLPQPDLRYLFLNGNKLARVAAGAFQGLRQLDMLDLSNNSLASVPEGLWASLGQPNWDMRDGFDISGNPWICDQNLSDLYRWLQAQKDKMFSQNDTRCAGPEAVKGQTLLAVAKSQ
Source
Humanpooledserum
Purity
Purityasdeterminedbydensitometricimageanalysis:>95%
SDS-PAGEGel
SDS-PAGEanalysisofhumanLeucine-richalpha-2-glycoprotein-1nativeprotein,12%gelstainedwithCoomassieBrillantBlueR250
- M.W.Marker-14,21,31,45,66,97kDa
- reducedandheatedsample,2.5μg/lane
- non-reducedandnon-heatedsample,2.5μg/lane
BIOLOGicalActivity
ProteinspecificallybindtohumanCytochromec1,hemeprotein –tested.
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0,4 μm)andlyophilizedin0.5 mg/mlin20mMTRIS,20mMNaCl,pH 8.0
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies,Immunologicalmethods
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
AllbloodsamplesusedforproteinpreparationweretestedandfoundnegativeforHBsAg,anti-HCV,HIVAg/Ab,andsyphilis.Sincenotestcanabsolutelyassuretheabsenceofallinfectiousagents,thisproductshouldbehandledasapotentialbiohazard.Thisproductisintendedforresearchuse only.
