Type

Recombinant

Description

IP-10 MouseRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining77 aminoacidsandhavingamolecularmassof8701 Dalton.TheIP-10 ispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeIle-Pro-Leu-Ala-Arg.

Source

E.coli

Purity

Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.

BIOLOGicalActivity

TheBiologicalactivityiscalculatedbyitsABIlitytochemoattractIL-2 activatedTcellsat0.1–10 ng/ml.

Formulation

TheCXCL10proteinwaslyophilizedwithnoadditives.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedIP-10 althoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionCXCL10shouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.