DESCRIPTION:
HumanrecombinantXIAP-BIR3.
ACCESSION#:NM_001167.3
UniprotLink
INCLUDESAMINOACIDS:255-356
TAG(S):N-terminalHistagC-terminalStrepIItag
MW:15.8kDa
EXPRESSIONSYSTEM:E.coli
PURITY:>95%bySDS-PAGE
ASSAYCONDITIONS:
RBC’sXIAP-BIR3domaindisplaysbindingaffinityforSmac/DIABLOpeptide.Reactionswereperformedwith20nMFAM-labelledpeptideatroomtemperaturein50mMHEPES,pH7.5,200mMNaCl,0.01%TritonX100.Theresultingpolarizationvalues(Ex.480nm/Em.535nm)wasreadfollowinga30minuteincubation.(Seefigure,below.)
SUPPLIEDAS:_µg/µLin50mMTrisHCl,pH7.5,500mMNaCl,1mMTCEP,10%glycerol
STORAGE:-70°C.Thawquicklyandstoreonicebeforeuse.Theremaining,unused,undilutedproteinshouldbesnapfrozen,forexampleinadryiceethanolbathorliquidnitrogen.Minimizefreeze/thawsifpossIBLe,butverylowvolumealiquots(<5µl)orstorageofdilutedenzymeisnotrecommended.
AMOUNT:100ug
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