BIX-01294 is a selectiveinhibitor of G9a histonemethyltransferase (G9aHMTase; IC50 = 1.7 µM) as wellas GLP HMTase (IC50 = 38 µM) leading to a decrease in H3K9Me2(histone H3 lysine 9 methylation) invitro. It facilitatesthe reactivation of pluripotency genes and induces passive demethylation, thuspromoting reprogramming.BIX-01294, in combination with BAY K8644 (a calcium channel agonist),was found to improve reprogramming efficiencies of Oct4-Klf4-(OK)-infectedneural progenitor cells. Itinduced apoptosis and decreased proliferation, mobility and invasion in humanneuroblastoma cells.
Physical Properties: Off-whitecrystalline powder
MW=600.0 C28H38N6O2· 3HCl
Analysis: >98% (HPLC); NMR(Conforms)
Solubilization: May be dissolved in water (50 mg/ml); or DMSO (50 mg/ml)
Storage: Store desiccated assupplied at ambient for up to 2 years. Store solutions at -20°C for up to 3 months.
Amount: 5mg