DESCRIPTION:Human recombinant TRIM33b PHD-bromodomain construct. A member of the Tripartite Motif (TRIM) family of proteins, TRIM33 functions as an E3 ubiquitin protein ligase for SMAD4. The C-terminally located PHD-bromodomain reader cassette displays a high affinity for histone H3 N-terminal tails with unmodified lysine-4, trimethylated lysine-9 (H3K9me3, bound by PHD) and acetylated lysine-18 (H3K18Ac, bound by bromodomain). The biological roles of TRIM33 include tumor suppression in various tissues, regulation of ALC1 activity in the PARP-dependent DNA damage response, transcriptional regulation in hematopoiesis and regulation of TGF-ß signaling in stem cell differentiation.
ACCESSION #:NM_033020
Uniprot Link
INCLUDES AMINO ACIDS:885-1074
TAG(S):N-terminal His tag
MW:24.6 kDa
EXPRESSION SYSTEM:E.coli
SUPPLIED AS:_ µg/µL in 50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v)
STORAGE:-70°C. Thaw quickly and store on ice before use. The remaining, unused, undiluted protein should be snap frozen, for example in a dry/ice ethanol bath or liquid nitrogen. Minimize freeze/thaws if possible, but very low volume aliquots (<5 µl) or storage of diluted protein is not recommended.
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Substantial Discounts Available on Bulk Quantities
