DESCRIPTION:NuclearExtract(HeLa)isahighsaltextractofnucleifromHeLa-S3cells(humancervicalcancerline).Suchextractscontainvariousenzymesandmulti-enzymecomplexeswithepigeneticmodificationactivities,suchashistonedeacetylases(HDACs)andhistonemethyltransferases(HMTs).RBC’sHeLaNuclearExtractdisplayshistonemethyltransferase(HMT)activityinrADIolabeledassayswith[3H]-S-adenosylmethionineandcorehistonesornucleosomesassubstratesanddeacetylase(HDAC)activitywithafluorogenicacetylatedlysinesubstrate.
SOURCE:HeLaS3cells
SUPPLIEDAS:Dialyzedandclarifiedproteinextractat7-10mg/mLin20mMHEPES/NaOHpH7.9,100mMKCl,0.2mMEDTA,0.5mMPMSF,0.5mMDTT,20%glycerol(v/v).
STORAGE:-80°C,aliquotandsnap-freezeafterfirstuse.
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