ProZyme/Glyko® Sialidase A™ [GK80040]/GK80040/1 U

价格
¥2662.40
货号:GK80040
浏览量:127
品牌:ProZyme
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商品描述
Description

Releases α(2,3)-,α(2,6)-,α(2,8)-,andα(2,9)-linkedN-acetylneuraminicacidfrom oligosaccharidesandglycoproteins. AlsocapableofreleasingN-glycolylneuraminicacid.

Source:
RecombinantgenefromArthrobacterureafaciens,expressedinE.coli.

Specificity:
Theenzymereleasesα(2,3)-,α(2,6)-,α(2,8)-,andα(2,9)-linkedN-acetylneuraminicacidfromcomplexcarbohydrates.Theinitialrateofhydrolysisofα(2,6)linkagesisreportedtobeapproximatelytwicethatofα(2,3)-linkedsialicacidhowever,inpractice,thiskineticselectivityisoflittleconsequenceduringextendedincubations.Effectivedigestionofglycolipidsubstratesisfacilitatedbyadditionofadetergent,suchassodiumtaurodeoxycholatetotheincubation.

SialidaseAiscapableofreleasingN-glycolylneuraminicacid(Neu5Gc,NGNA)inadditiontoN-acetylneuraminicacid(Neu5Ac,NANA)[1],althoughsimilarlytoothersialidases[2]theactivityislowertowardNeu5GcthanNeu5Ac.

Theenzymecanalsobeusedtoremovesialicacidfromgangliosides[3],glycosphingolipds(ceramide&oligosaccharide)withsialicacid.

MaybeusedforexoglycosidasesequencinginconjunctionwithSialidaseS(GK80021),whichisspecificforα(2,3)-linkedsialicacids.

Sialidasesarealsoknownasneuraminidases.

Shipswith:
WS00495xReactionBufferB[250mMsodiumphosphatepH6.0]

ReactionBuffer:
5Xconcentratedbufferwhichwhendilutedgives50mMsodiumphosphatepH6.0.

Formulation:
20mMTrisHClpH7.5,containing25mMNaCl.

pHoptimum:
6.0

UnitDefinition:
Oneunitisdefinedastheamountofenzymerequiredtocatalyzethereleaseof1μmoleofp-nitrophenolfromp-nitrophenyl-α-D-N-acetylneuraminicacidperminuteat37°C,pH5.5.

Size:1U(200µl)

Concentration: ≥5U/ml

ProductCode:GK80040

  1. UchidaY,TsukadaY,SugimoriT. EnzymaticpropertiesofneuraminidasesfromArthrobacterureafaciens. 1979Nov;86(5):1573-85.
  2. SchauerR. Chemistry,metabolism,andBIOLOGicalfunctionsofsialicacids. 1982;40:131-234.
  3. LarssonEA,OlssonU,WhitmoreCD,MartinsR,TettamantiG,SchnaarRL,DovichiNJ,PalcicMM,HindsgaulO. Synthesisofreferencestandardstoenablesinglecellmetabolomicstudiesoftetramethylrhodamine-labeledgangliosideGM1. 2007Feb26;342(3-4):482-9
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