Description

Releasesunsubstituted,Ser/Thr-linkedGalGalNAcfromproteins;usedwithadditionalenzymestoremovemorecomplexO-linkedstructures.Alsoknownas Endo-O-Glycosidase™,Endo-αN-acetylgalactosaminidase,O-Glycosidase. 

Source:
RecombinantgenefromStreptococcuspneumoniae,expressedinE.coli.

Specificity:
Theenzymeishighlyspecific,liberatingGalβ1-3GalNAcfromserineorthreonineresiduesaloneoraspartofglycopeptidesorglycoproteins.Thereisnoapparentpreferenceforserineoverthreonine.Substitutionofthedisaccharidecorewithsialicacid,fucose,N-acetylglucosamineorN-acetylgalactosamineresidues,preventscleavageoftheglycosylaminelinkage.AbsenceofthegalactoseresidueortheacetamidogroupontheinnermostN-acetylgalactosamineresiduealsopreventscleavageoftheglycosylaminelinkage.

O-Glycanaseisincludedinour EnzymaticDeglycosylationKitforN-Linked&SimpleO-LinkedGlycans(GK80110),whichmaybeusedinconjunctionwithour prO-LINKExtender™KitforComplexO-LinkedGlycans(GK80115).

Applications:

• DetectionofO-glycosylationinproteins
• Epitopeandbindingsiteanalysis
• BioactivityinO-glycans
• Analysisofbiosynthesisofglycoproteins
• StructuralanalysisofO-glycan
• Glycoproteindeglycosylation

Shipswith:
WS00595XreactionbufferA[250mMsodiumphosphatepH5.0]

Formulation:
20mMTrisHClpH7.5containing25mMNaCl.

ReactionBuffer:
5Xconcentratedbufferwhichwhendilutedgives50mMsodiumphosphatepH5.0.

MW:
~180kD

pHoptimum/range:
5.5/5.0-7.0

UnitDefinition:
Oneunitisdefinedastheamountofenzymerequiredtocatalyzethereleaseof1μmoleofGalβ1-3GalNAcα1-p-nitrophenolperminuteat37°C,pH5.5.

Size:50mU(40µl)

Concentration: ≥1.25U/ml

ProductCode:GK80090

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