Description
PNGaseF,peptide-N-glycosidaseF,peptide-N4-(N-acetyl-β-glucosaminyl)asparagineamidase. ReleasesintactN-glycansbycleaving betweentheinnermostGlcNAcandAsn. SamemanufacturingprocessasN-GlycanaseforGlykoPrep(EDTA-free),suppliedatthe GKE-5006 concentrationof ≥2.5U/ml.
Source:
RecombinantgenefromElizabethkingiameningosepticum,expressedinE.coli.Thesourceorganismwaspreviouslyknownas Chryseobacterium[Flavobacterium]meningosepticum[1].
Specificity:
CleavesallN-linkedcomplex,hybridorhighmannoseoligosaccharides,unlessα(1-3)corefucosylated,asinplantglycans.Asparaginemustbepeptide-bondedatbothtermini.Phosphate,sulfateandsialicacidgroupsattachedtotheoligosaccharidedonotaffectcleavage.EndoFfree.
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Figure1:CleavagespecificityforGKE-5016N-Glycanase(PNGaseF).
R1:N&CsubstitutionotherthanH
R2: maybeHortherestofanoligosaccharide
R3: maybeHor α(1-6)-linkedfucose
"A&B"packsizesshipwith:
- 1eaWS00105xN-GlycanaseReactionBuffer
- 1eaWS0012DenaturationSolution
- 1eaWS0013DetergentSolution
- 1eaWS01455xN-GlycanaseTrisReactionBuffer(MSapplications)
"D"packsizedoesnotshipwiththeabovebuffers. Buffersareavailablewith"D"packsizeonrequest.
Formulation:
20mMTris-HCl,50mMNaCl(pH7.5)
ReactionBuffer:
5Xconcentratedbuffer,whendilutedgives20mMsodiumphosphatepH7.5,containing0.02%sodiumazide.
MW:
~35,000Daltons
pHoptimum/range:
8.6/7.5-9.5
UnitDefinition:
OneunitisdefinedastheamountofenzymerequiredtocatalyzethereleaseofN-linkedoligosaccharidesfrom1μmoleofdenaturedribonucleaseBperminuteat37°C,pH7.5.
NotformulatedforFACE®analysis,useGK50050instead.
Size:1Uin400µl
Concentration: ≥2.5U/ml
ProductCode:GKE-5016D
1.KimKK,KimMK,LimJH,ParkHY,LeeST. TransferofChryseobacteriummeningosepticumandChryseobacteriummiricolatoElizabethkingiagen.nov.asElizabethkingiameningosepticacomb.nov.andElizabethkingiamiricolacomb.nov. IntJSystEvolMicrobiol.2005May;55(Pt3):1287-93.
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