Description

TheGly-X™N-GlycanRapidReleaseandLabelingwithInstantAB™kitutilizesanovelin-solutionenzymaticproteindeglycosylationfollowedbyrapidinstantlabeling ofreleasedN-glycanswithInstantAB dye.Withdeglycosylationandlabelingcarriedoutinsolution,themethodissimple,rapidandsuitableforautomation.Afterasimpleclean-upstep,theInstantAB-labeledglycansamplesarereadyforanalysisbyUHPLC,LC-MS,andothermethods. 

Samplepreptime: ~1hour.

Benefitsinclude:

• InstantlabelingwithInstantAB
• 5-minutePNGaseFdigestionprovidesunbiasedN-glycanrelease
• FlexIBLe,high-throughputformat:process1to96samples
• Optimizedcleanupremovesexcessfreedye,protein,andotherinterferingcompounds.

Contains:

• GX96-100Gly-XDeglycosylationModule
• GX96-501Gly-XInstantAB LabelingModule
• GX96-502Gly-XInstantABClean-upModule

Equipmentandreagentsprovidedbyuser:

• 96-wellThermocyclerortwoindependentheatblocks,setfor90°Cand50°C
• Thermocycler(CorningTHERM-1001,110V;THERM-1000,230V)
• Note:TwoGlykoPrepHeaters,WS0271,canbefittedwithVWR13259-260ModularHeatingBlocks
• Vacuummanifold(MilliporeMSVMHTS00-HTS)
• Vacuumpump(MilliporeWP6211560,110V;WP6122050,220V;WelchWOB-LPump2522)
• Acetonitrile(ACN),MS-grade(FisherA955-4recommended

Size:1kit(96-ct)

ProductCode:GX96-IAB

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