Description

TheGly-X™N-GlycanRapidReleaseandLabelingwithInstantQ™kitutilizesanovelin-solutionenzymaticproteindeglycosylationfollowedbyrapidlabelingofreleasedN-glycanswithInstantQdye.Afterasimpleclean-upstep,theglycansamplesarereadyforanalysisonthe Gly-QGlycanAnalysisSystem. Withdeglycosylationandlabelingcarriedoutinsolution,themethodissimple,rapidandsuitableforautomation.

Samplepreptime: ~1hour.

Otherbenefitsinclude:

• Instantfluorescentdyeoptimizedfor Gly-QGlycanAnalysisSystem
• FlexIBLe,high-throughputformat:process1to24 samples
• Optimizedcleanupremovesexcessfreedye,protein,andotherinterferingcompounds.

Contains:

• GX24-300Gly-XDeglycosylationModuleforInstantQ 
• GX24-301Gly-XInstantQLabelingModule
• GX96-302Gly-XInstantQClean-upModule

Equipmentandreagentsprovidedbyuser:

• 96-wellThermocyclerortwoindependentheatblocks,setfor90°Cand50°C
• Thermocycler(CorningTHERM-1001,110V;THERM-1000,230V)
• Note:TwoGlykoPrepHeaters,WS0271,canbefittedwithVWR13259-260ModularHeatingBlocks
• Vacuummanifold(MilliporeMSVMHTS00-HTS)
• Vacuumpump(MilliporeWP6211560,110V;WP6122050,220V;WelchWOB-LPump2522)
• Formicacid,MS-grade(FisherA117-50recommended)
• Acetonitrile(ACN),MS-grade(FisherA955-4recommended)

Size:1kit(24-ct)

ProductCode:GX24-IQ

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