Active Motif offers a wide variety of recombinant histones that include site- and degree-specific modifications such as methylation, acetylation and phosphorylation. The combination of histone post-translational modifications form the basis of the “histone code” that serves to regulate a variety of nuclear functions, including interactions with chromatin-associated proteins, nucleosome remodeling, transcriptional regulation, replication and DNA repair. Each recombinant histone is prepared using one of two patented technologies: Expressed Protein Ligation (EPL) or Methylated Lysine Analog (MLA). We also offer a subset of our histone H3 proteins that have been biotinylated for use in FRET assays and other capture techniques.
With our MLA technology, methylated histones are generated via a chemical alkylation reaction that introduces a methyl-lysine analog at the desired lysine location, giving us precise control over the site and degree of methylation. Alternatively, the EPL technology can be used to generate methylated, acetylated and phosphorylated histones. Using EPL, the histone globular domain is ligated to a peptide that contains the N-terminal histone tail with the desired modifications. This ligation reaction maintains the native histone bonds. Both methods produce proteins that are validated to be over 98% pure. For more information on the EPL and MLA technologies, click on the EPL and MLA Technologies tab below.
Active Motif also offers a number of biotinylated recombinant histone H3 proteins. Biotin is linked either to unmodified or modified recombinant H3 protein at its N-terminus via a carbon linker. The addition of biotin enables the recombinant histones to be used as a substrate for capture of protein binding interactions. Biotinylated histone H3 proteins are also ideal substrates for homogenous FRET assays. Simply incubate the recombinant histone with the enzyme of interest and detect using streptavidin-coated donor beads and antibody-conjugated acceptor beads.
A complete list of recombinant histones is shown below. Click on the protein name to see complete information.
其产品涉及：染色质免疫共沉淀检测试剂、DNA甲基化检测及收集试剂、组蛋白纯化试剂、检测组蛋白修饰的抗体、组蛋白乙酰转移酶活性检测试剂、核蛋白抽提试剂、有丝分裂指数分析试剂及转录因子ELISA试剂等。 产品列表： 53033 40 rxns (1 ml) 53034 200 rxns (5 ml) 11096 1 x 96 rxns 11596 5 x 96 rxns 13001 1 array 13005 5 arrays 13006 5 rxns 13007 5 rxns 14001 50 µl 14021 5 g 14022 500 mg 14023 100 mg 14024 25 mg 14025 5 mg 14026 50 mg 14027 10 mg 14028 25 mg 14029 5 mg 14033 1 g 14034 50 mg 14035 10 mg 14036 25 mg 14037 5 mg 14038 5 mg 14039 1 mg 14040 5 mg 14041 1 mg 14042 25 mg 14043 5 mg 14044 25 mg 14045 5 mg 14046 250 mg 14047 50 mg 14048 25 mg 14049 5 mg 14051 200 µg 14052 25 mg 14053 5 mg
