Active Motif蚂蚁淘在线

Applications

ApplicationNotes

ValidatedApplications:
DB:0.1-1µg/mldilution

PublishedApplications:
ICC/IF
DotBlot
Pleaseseereferencesformoreinformation.Individualoptimizationmayberequired.

Immunogen

This3-methylcytosineantibodywasraisedagainst3-methylcytidineconjugatedtoKLHandrecognizes3-methylcytosine.

Buffer

PurifiedrabbitIgGinPBSwith30%glyceroland0.035%sodiumazide.Sodiumazideishighlytoxic.

References

3-Methylcytosine (3-mC) antibody (pAb) tested by dot blot analysis.

3-Methylcytosine(3-mC,3-methylcytidine)antibodytestedbydotblotanalysis.
BSAconjugatednucleosides(startingat10ngasindicated)werespottedontoPVDFmembraneandblottedwith3-methylcytidineantibodyatadilutionof1:10,000.
Lane1:3-methylcytidine.
Lane2:Cytidine.
Lane3:5-methylcytidine.
Lane4:5-hydroxymethylcytidine.

Background

MethylationofDNAcanoccurnon-enzymaticallyatthenitrogen-threeofthecytosinebasethroughspontaneousexposuretoendogenousS-adenosylmethionine(SAM).Theresulting3-methylcytosine(3-mC)ismutagenicandmustberepaired,whichoccursinhumansthroughthebaseexcisionrepair(BER)ordealkylationviahumanhomologuesoftheE.coliAlkBprotein.3-methylcytosineispresentinhumancelllinesandincreasedlevelsof3-mCimpairproliferation.

CompatIBLeSecondaryAntibodies

ActiveMotifhasdevelopedavarietyofhigh-qualitysecondaryantibodiesformostapplications,withanti-rabbitandanti-mouseconjugatestoabroadlineofhigh-qualityfluorescentmolecules,aswellashorserADIshperoxidase(HRP).VisitourSecondaryAntibodyConjugatespagetofindtheperfectsecondariesforimmunofluorescenceexperimentswith3-Methylcytosine(3-mC)antibody(pAb).

Storage

Someproductsmaybeshippedatroomtemperature.ThiswillnotaffecttheirstABIlityorperformance.Uponreceipt,unconjugatedantibodiesmaybestoredat-20°Cforupto2years.Fluorophore-&enzyme-conjugatedantibodiesshouldbestoredat4°C.Fluorophore-conjugatedantibodiesshouldbeprotectedfromlight.Keepreagentsonicewhennotinstorage;toavoidrepeatedfreeze/thawcycles,werecommendaliquotingitemsthatwillbestoredfrozenintosingle-usefractionspriortofreezing.

Guarantee

Thisproductisguaranteedfor6monthsfromdateofreceipt.

Thisproductisforresearchuseonlyandisnotforuseindiagnosticprocedures.

ApplicationKey

ChIP=ChromatinImmunoprecipitation;
DB=DotBlot;
ELISA=Enzyme-linkedImmunosorbentAssay;
EMSA=ElectrophoreticMobilityShiftAssay
FC=FlowCytometry;
ICC=Immunocytochemistry;
IF=Immunofluorescence;
IHC=Immunohistochemistry;
IP=Immunoprecipitation;
MeDIP=Methyl-DNAImmunoprecipitation;
TR-FRET=Time-ResolvedFluorescenceResonanceEnergyTransfer;
WB=WesternBlotting

其产品涉及：染色质免疫共沉淀检测试剂、DNA甲基化检测及收集试剂、组蛋白纯化试剂、检测组蛋白修饰的抗体、组蛋白乙酰转移酶活性检测试剂、核蛋白抽提试剂、有丝分裂指数分析试剂及转录因子ELISA试剂等。产品列表： 53033 40 rxns (1 ml) 53034 200 rxns (5 ml) 11096 1 x 96 rxns 11596 5 x 96 rxns 13001 1 array 13005 5 arrays 13006 5 rxns 13007 5 rxns 14001 50 µl 14021 5 g 14022 500 mg 14023 100 mg 14024 25 mg 14025 5 mg 14026 50 mg 14027 10 mg 14028 25 mg 14029 5 mg 14033 1 g 14034 50 mg 14035 10 mg 14036 25 mg 14037 5 mg 14038 5 mg 14039 1 mg 14040 5 mg 14041 1 mg 14042 25 mg 14043 5 mg 14044 25 mg 14045 5 mg 14046 250 mg 14047 50 mg 14048 25 mg 14049 5 mg 14051 200 µg 14052 25 mg 14053 5 mg

Active Motif（艾跃）/3-Methylcytosine (3-mC) antibody (pAb)/61112/10µg