ThehumanlatentTGF-β1ELISAdevelopmentkitisidealforuserswhowanttheflexibilityofsettinguptheirownELISAassay.ThisproductenablesquantificationofhumanlatentTGF-β1insolution,e.g.incellculturesupernatant.Thekitcontainsamatchedpairofmonoclonalcaptureanddetectionantibodies,Streptavidin-ALPandELISAstandard.Platesandsubstratearenotincluded.
TGF-β1issecretedinalatent,BIOLOGicallyinactiveform,non-covalentlylinkedwithaLatencyAssociatedProtein(LAP).ThemonoclonalantibodiesMT593andMT517-biotinbindtotheLAPentityofhumanlatentTGF-β1.ThisenablesdirectquantificationoflatentTGF-β1inELISAwithnoneedofsamplepre-treatment.TheELISAdoesnotdetecthumanlatentTGF-β2or-β3orbovinelatentTGF-β1.
HumanlatentTGF-β1ELISAdevelopmentkitsarealsoavailablewithStreptavidin-HRP.SeeRelatedproducts.
Serum/Plasmasamples
Analysisofserum/plasmasampleswiththiskitrequirestheuseofELISAdiluentfordilutionofthesamples,standardanddetectionantibody.Thediluentpreventsfalsepositiveread-outswhichmaybecausedbyinterferenceofheterophilicantibodiesfoundinserumandplasma.TheELISAdiluentisavailablefromMabtech.SeeRelatedproducts.
Speciescross-reactivity
Themonoclonalantibodiesinthiskitcross-reactwithlatentTGF-β1fromnon-humanprimates.SeetheHuman/NHPcross-reactivitytable.
ELISAsubstrate
Ready-to-useELISAsubstrateisavailableinourwebshop.SeeRelatedproducts.
AnalyteInformation
TGF-β1(LatentTGF-β1)
TransformingGrowthFactorβ1(TGF-β1)issecretedinalatent,biologicallyinactiveform,non-covalentlylinkedwithaLatencyAssociatedProtein(LAP).ThemonoclonalantibodiesMT593andMT517-biotinbindtotheLAPentityofhumanlatentTGF-β1.ThisenablesdirectquantificationoflatentTGF-β1inELISAwithnoneedofsamplepre-treatment.TheELISAdoesnotdetecthumanlatentTGF-β2or-β3orbovinelatentTGF-β1.
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Standardrange: | 0.5-50pM |
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Size: | for6plates |
Otherformats: | ClicktoviewallHuman/NHPTGF-β1(LatentTGF-β1)products |
SavetimeandreducevariABIlitywiththe completeandpre-coated3kit | |
Mabtechserviceishighlycustomer-friendlyandefficient.WehavebeenusingMabtechELISAkitsforthepasteightyearswithfullsatisfaction.FurThermore,IfindMabtechproductsthebestforevendevelopingmyin-houseELISA. Mabtech提供了多种不同格式的ELISpot试剂盒,用于检测非人类灵长类动物(NHP)样品中的细胞因子和其他免疫效应分子。
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