TheapoBELISAdevelopmentkitisidealforuserswhowanttheflexibilityofsettinguptheirownELISAassay.ThisproductenablesquantificationofapoBinsolution,e.g.incellculturesupernatants.TheassayisspecificforapoB100anddoesnotrecognizeapoB48.Thekitcontainsamatchedpairofmonoclonalcaptureanddetectionantibodies,Streptavidin-ALPandELISAstandard.Platesandsubstratearenotincluded.
ApoBELISAdevelopmentkitsarealsoavailablewithStreptavidin-HRP.SeeRelatedproducts.
Serum/Plasmasamples
Analysisofserum/plasmasampleswiththiskitrequirestheuseofApoELISAbufferfordilutionofthesamples,standardanddetectionantibody.Thebufferpreventsfalsepositiveread-outswhichmaybecausedbyinterferenceofheterophilicantibodiesfoundinserumandplasma.TheApoELISAbufferisavailableasa5xconcentratedsolutionfromMabtech.SeeRelatedproducts.
Dilutionguidelinesforserum/plasmasamplescanbefoundhere.
Speciescross-reactivity
Themonoclonalantibodiesinthiskitcross-reactwithApoBfromnon-humanprimates.SeetheHuman/NHPcross-reactivitytable.
ELISAsubstrate
Ready-to-useELISAsubstrateisavailableinourwebshop.SeeRelatedproducts.
AnalyteInformation
ApoB
ApolipoproteinB(apoB,alsoknownasapolipoproteinB,apoB,apoB-100,apoB100andapoB100)isthemainproteincomponentoflowdensitylipoproteinparticles(LDL)anditisalargewaterinsolublemoleculeof512kD(apoB-100)producedmainlyintheliverandintheintestine(apoB-48).ApoBisregardedasanegativefactorincardiovasculardisease.ApoB-100isnormally98%oftheapoBinhumanplasmaandataconcentrationofapproximately0.4-1.3mg/ml.
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Standardrange: | 8-800ng/ml |
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Size: | for6plates |
Otherformats: | ClicktoviewallHuman/NHPapoBproducts |
SavetimeandreducevariABIlitywiththe completeandpre-coated3kit | |
Mabtechserviceishighlycustomer-friendlyandefficient.WehavebeenusingMabtechELISAkitsforthepasteightyearswithfullsatisfaction.FurThermore,IfindMabtechproductsthebestforevendevelopingmyin-houseELISA. Mabtech提供了多种不同格式的ELISpot试剂盒,用于检测非人类灵长类动物(NHP)样品中的细胞因子和其他免疫效应分子。
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