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蚂蚁淘在线 / 品牌中心 / Adipogen / Adipogen/Di-ubiquitin [Ub2] (K33-linked) (human) (rec.)/AG-40T-0237-C025/25 µg
商品描述
| ProductDetails | |
|---|---|
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | Twohumanubiquitinmonomers(AccessionNr.P0CG47)covalentlylinkedthroughanisopeptidebondattheK33residueofoneubiquitinmoleculeandtheC-terminalglycineresidueofanotherubiquitinmolecule. |
| Crossreactivity | Human |
| MW | ~17kDa |
| Purity | ≥95%(SDS-PAGE) |
| Concentration | 1mg/ml |
| Formulation | Liquid.Insterile,deionizedwater. |
| OtherProductData | Use:Formedwithwild-typehumanrecombinantubiquitinandlinkage-specificenzymes.ValidatedbyUb-AQUA,anLCMS-basedtechniquethatprovidesextremelyaccurateinformationonthecompositionofPoly-ubiquitinsamples.Idealforinvestigatingubiquitin-bindingproteinsandassubstratesforubiquitin-specificisopeptidases.Reactionconditionswillneedtobeoptimizedforeachspecificapplication. IMPORTANT:HeatingthisproductinSDS-PAGEbufferorterminatingreactionscontainingthisproductwithheatedSDS-PAGEbuffercouldleadtounexpected,highapparentmolecularweightbandingorsmearingongelsthatisnotrepresentativeofproductpurity.Foroptimalresults,werecommendincubationinSDS-PAGEbuffer+DTTat<40°c for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.=""> |
| Declaration | ManufacturedbyBostonBiochem |
| ShippingandHandling | |
| Shipping | DRYICE |
| ShortTermStorage | -20°C |
| LongTermStorage | -80°C |
| HandlingAdvice | Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-80°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
Withapredictedmolecularweightof17kDa,di-ubiquitiniscomposedoftwoubiquitinmonomersthatarecovalentlylinkedthroughanisopeptidebond,whichtypicallyformbetweenalysineresidueofoneubiquitinmoleculeandtheC-terminalglycineresidueofanotherubiquitinmolecule.Eachhumanubiquitinmonomeris76aminoacids(aa)inlengthandshares96%and100%aaidentitywithyeastandmouseubiquitin,respectively.Ubiquitinhassevenlysineresiduesthatcanparticipateintheformationofpoly-ubiquitinchains.Thespecificlysineresidueusedinubiquitinconjugationisthoughttodeterminethefunctionofpoly-ubiquitinationincellularprocessessuchasproteindegradation,signalingandtrafficking.
Linkage-specificdi-ubiquitinisasubstrateforenzymesthatcleavetheisopeptidelinkagebetweentwoubiquitinmolecules.ItcanalsobeusedtoinvestigatemechanismofbindingandrecognitionbyE1orE2enzymes,deubiquitinatingenzymes,E3ligasesorotherproteinsthatcontainubiquitin-associateddomainsorubiquitin-interactingmotifs(UIMs).
Linkage-specificdi-ubiquitinisasubstrateforenzymesthatcleavetheisopeptidelinkagebetweentwoubiquitinmolecules.ItcanalsobeusedtoinvestigatemechanismofbindingandrecognitionbyE1orE2enzymes,deubiquitinatingenzymes,E3ligasesorotherproteinsthatcontainubiquitin-associateddomainsorubiquitin-interactingmotifs(UIMs).
