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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
| ProductDetails | |
|---|---|
| ProductType | Protein |
| Properties | |
| Source/Host | CHOcells |
| Sequence | TheextracellulardomainofmouseCD28(aa21-149)isfusedtotheN-terminusoftheFcregionofmouseIgG2a. |
| Crossreactivity | Mouse |
| Purity | ≥98%(SDS-PAGE) |
| EndotoxinContent | <0.06eu mu;g="" protein="" (lal="" test;="" lonza).=""> |
| Reconstitution | Reconstituteat100μg/mlinsterilePBS. |
| Formulation | Lyophilizedfrom0.2μm-filteredsolutioninPBS. |
| OtherProductData | NCBIreferenceNP_031668.3:CD28(mouse) |
| Declaration | ManufacturedbyChimerigen. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Avoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-20°C. Workingaliquotsarestableforupto3monthswhenstoredat-20°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
CD28andCTLA-4togetherwiththeirligands,CD80(B7-1)andCD86(B7-2),constituteoneofthedominantcostimulatorypathwaysthatregulateTandBcellresponses.CD28andCTLA-4arestructurallyhomologousmoleculesthataremembersoftheimmunoglobulin(Ig)genesuperfamily.BothCD28andCTLA-4arecomposedofasingleIgV-likeextracellulardomain,atransmembranedomainandanintracellulardomain.CD28andCTLA-4arebothexpressedonthecellsurfaceasdisulfide-linkedhomodimersorasmonomers.Thegenesencodingthesetwomoleculesarecloselylinkedonhumanchromosome2andmousechromosome1.MouseCD28isexpressedconstitutivelyonvirtually100%ofmouseTcellsandondevelopingthymocytes.CellsurfaceexpressionofmouseCD28isdownregulateduponligationofCD28inthepresenceofPMAorPHA.Incontrast,CTLA-4isnotexpressedconstitutivelybutisupregulatedrapidlyfollowingTcellactivationandCD28ligation.CellsurfaceexpressionofmouseCTLA-4peaksapprox.y48hoursafteractivation.AlthoughbothCTLA-4andCD28canbindtothesameligands,CTLA-4bindstoB7-1andB7-2witha20-100foldhigheraffinitythanCD28.CD28/B7interactionhasbeenshowntopreventapoptosisofactivatedTcellsviatheupregulationofbcl-XL.CD28ligationhasalsobeenshowntoregulateTh1/Th2differentiation.
