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蚂蚁淘在线 / 品牌中心 / Adipogen / Adipogen/Ubiquitin Conjugation Reaction Buffer (10X)/AG-10T-0017-L005/5 ml
商品描述
| ProductDetails | |
|---|---|
| ProductType | Chemical |
| Properties | |
| Formulation | Liquid. |
| Crossreactivity | Multi-species |
| Declaration | ManufacturedbyBostonBiochem |
| OtherProductData | Use:UbiquitinreactionbuffercontainstheoptimalformulationforthechargingofubiquitinbyE1,thefirstreactionintheconjugationcascade.ThisE1-UbiquitinthioestercanbesubsequentlyusedfortheinitiationofanyE1mediatedreactionincludingE2ubiquitinthioesterformationand/ortheubiquitinationconjugationreactionoftargetsubstrateproteinswiththeadditionofE2/E3enzymes.ThisbuffercansupplementthefractionIIHeLaconjugationandfractionIIreticulocyteconjugation,ubiquitin-proteinconjugation,andubiqiutinthioester/conjugationkits.Diluteto1Xinfinalreaction. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Aliquottoavoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Ubiquitinisa76aminoacid(aa)proteinthatisubiquitouslyexpressedinalleukaryoticorganisms.ubiquitinishighlyconservedwith96%aasequenceidentitysharedbetweenhumanandyeastubiquitin,and100%aasequenceidentitysharedbetweenhumanandmouseubiquitin.Inmammals,fourubiquitingenesencodefortwoubiquitin-ribosomalfusionproteinsandtwopoly-ubiquitinproteins.Cleavageoftheubiquitinprecursorsbydeubiquitinatingenzymesgivesrisetoidenticalubiquitinmonomerseachwithapredictedmolecularweightof8.6kDa.ConjugationofubiquitintotargetproteinsinvolvestheformationofanisopeptidebondbetweentheC-terminalglycineresidueofubiquitinandalysineresidueinthetargetprotein.Thisprocessofconjugation,referredtoasubiquitinationorubiquitylation,isamulti-stepprocessthatrequiresthreeenzymes:aubiquitin-activating(E1)enzyme,aubiquitin-conjugating(E2)enzyme,andaubiquitinligase(E3).ubiquitinationisclassicallyrecognizedasamechanismtotargetproteinsfordegradationandasaresult,ubiquitinwasoriginallynamedATP-dependentProteolysisFactor1(APF-1).Inadditiontoproteindegradation,ubiquitinationhasbeenshowntomediateavarietyofBIOLOGicalprocessessuchassignaltransduction,endocytosis,andpost-endocyticsorting.
