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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
| ProductDetails | |
|---|---|
| ProductType | Chemical |
| Properties | |
| Formulation | Liquid. |
| Crossreactivity | Multi-species |
| Declaration | ManufacturedbyBostonBiochem |
| OtherProductData | Use:SUMOreactionbuffercontainstheoptimalformulationfortheuseinconjugationassaysoftheubiquitin-likemodifiers,SUMO1,SUMO2,andSUMO3,toproteinsubstratesinvitro.TheseSUMOproteinsareconjugatedtoavarietyofproteinsinthepresenceofUbc9andSAE1/SAE2(human).ThisbuffercanbeasupplementtoSUMOconjugationkits.Diluteto1Xinfinalreaction. |
| ShippingandHandling | |
| Shipping | BLUEICE |
| ShortTermStorage | +4°C |
| LongTermStorage | -20°C |
| HandlingAdvice | Aliquottoavoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-20°C. |
| Documents | |
| MSDS |  DownloadPDF |
| ProductSpecificationSheet | |
| Datasheet | DownloadPDF |
Smallubiquitin-likeModifiers(SUMOs)areafamilyofsmall,relatedproteinsthatcanbeenzymaticallyattachedtoatargetproteinbyapost-translationalmodificationprocesstermedSUMOylation.TherearefourknownSUMOs(SUMO1-4).AllSUMOproteinsshareaconservedubiquitindomainandaC-terminaldiglycinecleavage/attachmentsite.FollowingcleavageofaC-terminalprosegment,theC-terminalglycineresidueofSUMOisenzymaticallyattachedtoalysineresidueonatargetprotein.Inhumans,SUMOisconjugatedtoavarietyofmoleculesinthepresenceoftheSAE1/UBA2SUMO-activating(E1)enzymeandtheUBE2I/Ubc9SUMO-conjugating(E2)enzyme.Inyeast,theSUMO-activating(E1)enzymeisAos1/Uba2p.SUMOylationcanoccurwithouttherequirementofaspecificSUMOligase(E3),whereSUMOistransferreddirectlyfromUBE2I/Ubc9tospecificsubstrates.UnlikeSUMO1,whichisusuallyconjugatedtoproteinsasamonomer,SUMO2andSUMO3areknowntoformhighmolecularweightpolymersonproteins.SUMOprecursorprocessinganddeconjugationarecatalyzedbyafamilyofcysteineproteasesknownasSUMO-specificproteases(SENPs)andDeSUMOylatingIsopeptidase1.
