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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
| ProductDetails | |
|---|---|
| ProductType | Protein |
| Properties | |
| Source/Host | E.coli |
| Sequence | Humanubiquitin(aa1-76)(AccessionNr.P0CG47)withaC-terminalvinylsulfonegroup. |
| Crossreactivity | Human |
| Application | Typicalworkingconcentrationrangeis1-5μM.Reactionconditionswillneedtobeoptimizedforeachspecificapplication. |
| MW | ~9kDa |
| Purity | ≥97%(LCMS) |
| Concentration | Lotdependent. |
| AccessionNumber | P0CG47 |
| Formulation | Liquid.In50mMMESpH6.0,100mMsodiumchloride. |
| OtherProductData | ClickhereforaTypicalLot-specificProductDatasheetfromtheOriginalManufacturer Ourproductdescriptionmaydifferslightlyfromtheoriginalmanufacturersproductdatasheet. |
| Declaration | ManufacturedbySouthBayBio. |
| ShippingandHandling | |
| Shipping | DRYICE |
| ShortTermStorage | -20°C |
| LongTermStorage | -80°C |
| HandlingAdvice | Aliquottoavoidfreeze/thawcycles. |
| Use/StABIlity | Stableforatleast1yearafterreceiptwhenstoredat-80°C. |
| Documents | |
| MSDS | No |
| ProductSpecificationSheet | |
| Datasheet |  DownloadPDF |
Ubiquitinisasmall(8.5kDa)regulatoryproteinthathasbeenfoundinalmostalltissuesofeukaryoticorganisms.Theadditionofubiquitintoasubstrateproteiniscalledubiquitinationorubiquitylation.Ubiquitinationcanaffectproteinsinmanyways:itcansignalfortheirdegradationviatheproteasome,altertheircellularlocation,affecttheiractivityandpromoteorpreventproteininteractions.Removalofubiquitinfromasubstrateproteinoccursviadeconjugatingenzymes,ofwhichtherearenearly100knownenzymeswithvariouslinkagespecificities.Thisproductconsistsofafull-lengthhuman,matureubiquitinpolypeptide(aminoacids1-76),expressedinE.coliwithaC-terminalwarhead(VinylSulfone).Ubiquitinvinylsulfoneisapotent,irreversIBLeandspecificinhibitorofdeubiquitinatingenzymes(DUBs)basedonaC-terminalelectrophilicvinylsulfonegroup.UbiquitinvinylsulfonecanbeusedforactivityprofilingexperimentsanddeterminingDUBinhibitorspecificity.IttargetsthreeofthefourmajorDUBfamilies:UCH(UbiquitinC-terminalhydrolases),USP(Ubiquitinspecificproteases),OTU(Ovariantumorproteases)andMJD(Machado-Josephindomainproteases)whileJAMMmetalloproteasesarenotinhibited.
