Macerozyme R-10 is a macerating enzyme from the Rhizopus sp. which is perfectly suited for the isolation of plant cells.Macerozyme R-10 is often used in combination with Cellulase "Onozuka R-10" in the isolation of plant protoplasts.

Macerozyme R-10 is a multi-component enzyme mixture containing the following characteristics:

Enzyme activity: >3,000U/gPectinase: 0.5U/mgCellulase:0.1U/mg Hemicellulase:0.25U/mg Loss on drying: <10% solubility:1 mg/ml0.1M Sodium acetate buffer pH4.5 store at 2-8°C pH optimum:3.5-7.0

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。