Biotin‐NHS ester reagent enables simple and efficient biotin labeling of amine-containing organic molecules, antibodies, proteins and any other primary amine-containing biomolecules in solution. NH2-activated biotins require an organic solvent such as DMSO or DMF to be dissolved.

Formula: C₁₄H₁₉N₃O₅S

MW: 341.38 g/mol

Storage/Handling: Store at -20°C. Product shipped at ambient temperature. Soluble in DMSO or DMF

Highlights:- Biotin‐NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via a stable amide bond. - Biotin‐NHS ester is water insoluble - must be dissolved in DMSO or DMF before further dilution in aqueous buffers. - Spacer arm is 13.5 angstroms. - High purity, crystalline biotin labeling reagents can be used to create high-quality biotin-labeled derivatives biotinylation reagents.

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。