Low Melting (LM) Agarose is a low melting temperature agarose with the highest resolving capacity for large DNAfragments, including PCR products. Low melting temperature allows for the recovery of undamaged nucleic acids below denaturation temperature. Low gellingtemperature ensures In-Gel applications can be performed inremelted agarose, avoiding difficult DNA extraction steps.
Low Melting Agarose is ideal for digestion by agarose enzymes, which makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.
Technical SpecificationsLow Melt Agarose, for applications requiring low gel/melt temperature.
Molecular Biology Grade
EEO (Electroendomosis): ≤0.12Sulfate: ≤0.12%Gel Strength (1%): ≥500 g/cm2Gelling Temperature: 26±1.5°CMelting Temperature: ≤65.5°CDNase/RNase Activity: None DetectedDNA Resolution ≥1000 bp: Finely ResolvedGel Background: Very LowDNA Binding: Very Low
★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等,通常使用的是 GFP、EGFP、RFP(DsRed)等;荧光染料标记适用于标记抗体、多肽、小分子药物等;常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记(新的标记方法)适用于标记肿瘤,主要应用在活细胞实时动态荧光观察与成像;量子点(quantum dot) 是一种能发射荧光的半导体纳米微晶体,外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍,稳定性强 100 倍以上,具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调,并且光化学稳定性高,不易分解等诸多优点。