GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!
RecombinantMurineDefensin,Beta 3BD3, DEFB-3, MBD-3, DEFB103A
Murine beta-defensin 3 (DEFB3) is an antimicrobial peptide that contributes to the innate and adaptive immune systems and is active against gram-negative and gram-positive bacteria, fungi, and viruses. Like the other β-defensins, DEFB3 is a small protein that contains a motif consisting of six cysteine residues which form three intramolecular disulfide bridges. It is expressed in epithelial tissue of many organs, including the skin and lungs, and in leukocytes. Expression of DEFB3 is induced by proinflammatory cytokines such as IL1B and IFNG. Expression of murine DEFB3 was found to be downregulated in the epidermis by psychological stress due to decreased glucocorticoid production and subsequent decreased defensin-encapsulating lamellar body secretion. DEFB3 is a cationic peptide and interacts with the membranes of invading microbes, which are negatively charged due to the presence of lipopolysaccharides (LPS) or lipoteichoic acid (LTA). LTA and LPS have higher affinity for DEFB3 than for Ca+2 and Mg+2 ions. The larger defensin molecule displaces the smaller ion, changing the membrane structure and affecting the stability of the membrane; this can lead to the formation of pores and subsequent depolarization or lysis. Inhibition of DEFB3 by high salt concentration may play a role in the pathogenesis of cystic fibrosis.
Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.
Source: Escherichia coli
MW: ~4.6 kDa (41 aminoacids)
Purity: >95% by SDS-PAGEand HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E. coli is less than 20 μg/ml, corresponding to a specific activity of >50 IU/mg.
Storage/Handling: Store desiccated at-20°C.
MGI: 1351612
NOT FOR HUMAN USE!
Additional resources for this protein may be found at:OMIM
★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。
