GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Human NOGNoggin

Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β (Transforming Growth Factor) family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Noggin has been shown to modulate the activities of other BMPs including BMP2, 7, 13, and 14. Targeted deletion of noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system.Transgenic mice over-expressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation and severe osteoporosis.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture.

Sequence: MQHYLHIRPAPSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQLLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVGSCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

MW: ~46.2 kDa (2x206 amino acid) (A non-disulfide linkedhomodimer consisting of two 206 amino acid polypeptide chains)

Purity: >95% by SDS-PAGEand HPLC analyses

Biological Activity: Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ is 0.04-0.2 μg/mL in the presence of 50 ng/mL of recombinant human BMP-4.

Accession Number: Q13253

HGNC ID: 7866

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in aqueous buffer containing 10mM HAc to a concentration of0.1-1.0 mg/mL. Reconstituted solutions are stable for up to one week at2-8°C.Stock solutions should bealiquoted and stored at -80°C.Furtherdilutions should be made in appropriate buffered solutions.

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OMIM

NOT FOR HUMAN USE!

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