GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Recombinant Human Bone Morphogenetic Protein 2BMP2BMP2a

Bone Morphogenetic Proteins (BMPs) belong to the TGF-β (Transforming Growth Factor) superfamily of structurally related signaling proteins.BMP2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues; including the lung, spleen, brain, liver, prostate, ovary and small intestine.The functional form of BMP2 is a 26kDa protein composed of two identical 115 amino acid polypeptide chains linked by a single disulfide bond.

Formulation: Sterile filtered whitelyophilized powder. Purified and tested for use in cell culture.

Sequence: MQAKHKQRKRLKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACCVPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

MW: ~26.0 kDa (2x115 amino acid) ) (A homodimeric protein consisting oftwo 115 amino acid non-glycosylated polypeptide chains)

Purity: >95% by SDS-PAGEand HPLC analyses

Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of >5.0 × 10³ IU/mg.

Accession Number: P12643

HGNC ID: 1069

Source: Escherichia coli

Storage/Handling: This lyophilizedpreparation is stable at 2-8°C, but should be kept at -20°C for long termstorage. The reconstituted sample can be apportioned into working aliquots andstored at -80 °C for up to 6 months. Avoid repeated freeze/thaw cycles.

Reconstitution: The sample shouldbe briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitutein a siliconized tube using distilled water that contains a 0.1% BSA to aconcentration of 0.1-1.0 mg/mL. Reconstituted solutions are stable for up toone week at 2-8°C. Stock solutions should be aliquoted and stored at -80°C.Further dilutions should be made in appropriate buffered solutions.

Additional resources for this product may be found at:

OMIM

NOT FOR HUMAN USE!

Note: 1000 µg volume may be delivered in multiple containers.

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。