PIPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1 – 7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay. Solubility of PIPES increases when the free acid is converted to the sodium salt.

Buffers are made by adding base solution to PIPES free acid and titrating to the desired pH.

Product SpecificationsPIPES, Free Acid2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid

HIGH PURITY GRADE

Formula: C₈H₁₈N₂O₆S₂

MW: 302.37 g/mol

Storage/Handling: Store in a dry and well ventilated place.

PubChem Chemical ID: 79723

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