Mix-n-Stain™ CF750 Antibody Labeling Kit is a near-infrared fluorescent dye that is the brightest fluorescent dye available in its spectrum. Antibody conjugates prepared from CF™750 have by far the best signal-to-noise ratio than antibody conjugates prepared from other spectrally similar dyes.

Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your primary antibody with biotin or one of our superior CF™ dyes in 30 minutes without the need for a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF™dyes are highly water soluble, small organic dyes designed for labeling proteins and nucleic acids. Many of our CF™ dyes are brighter and more photostable than competing dyes. Click the link for a complete list of more than 20 GoldBio CF™ antibody labeling kits.

Note: We do not recommend using CF™750 labeled primary antibodies for use with the APC-Cy7 channel. The excitation wavelength for the APC-Cy7 channel is suboptimal for CF™750 and direct immunofluorescence staining using primary antibodies labeled with CF™750 generally results in very low signal.

Technical Summary Abs/Em Maxima: 755/777 nmExcitation laser line: 633 nm, 635 nm, 680 nm or 685 nmExtinction Coefficient: 250,000MW: ~3009 g/mol

Kit Components DyeMix-n-Stain™ 10X reaction bufferMix-n-Stain™ antibody storage bufferUltrafiltration vial

Storage/Handling: Store the kit at -20°C.

Product may be shipped on room temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。