Mix-n-Stain™ CF680 Antibody Labeling Kit is an outstanding near-IR, cyanine-based dye excitable at about 680 nm with emission at about 700 nm. CF™680 is excellent for labeling antibodies, producing the brightest fluorescence and highest signal-to-noise ratio in immunostaining among spectrally similar dyes. However, because of its relatively large molecular size, CF™680 is not recommended for labeling nucleic acids or relatively small biomoleMix-n-Stain™ CF680R Antibody Labeling Kit (Catalog # CF-860R) is better suited.

Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your primary antibody with biotin or one of our superior CF™ dyes in 30 minutes without the need for a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF™dyes are highly water soluble, small organic dyes designed for labeling proteins and nucleic acids. Many of our CF™ dyes are brighter and more photostable than competing dyes. Click the link for a complete list of more than 20 GoldBio CF™ antibody labeling kits.

Technical Summary Abs/Em Maxima: 681/698 nmExcitation laser line: 680 nm or 685 nmExtinction Coefficient: 210,000MW: ~3241 g/mol

Kit Components DyeMix-n-Stain™ 10X reaction bufferMix-n-Stain™ antibody storage bufferUltrafiltration vial

Storage/Handling: Store the kit at -20°C.

Product may be shipped on room temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

★活体技术荧光检测荧光蛋白标记适用于标记细胞、病毒、基因等，通常使用的是 GFP、EGFP、RFP（DsRed）等；荧光染料标记适用于标记抗体、多肽、小分子药物等；常用的有 Cy3、Cy5、Cy5.5 及 Cy7量子点标记（新的标记方法）适用于标记肿瘤，主要应用在活细胞实时动态荧光观察与成像；量子点（quantum dot) 是一种能发射荧光的半导体纳米微晶体，外观恰似一极小的点状物。量子点荧光比有机荧光染料的发射光强的 20 倍，稳定性强 100 倍以上，具有荧光发光光谱较窄、量子产率高、不易漂白、激发光谱宽、颜色可调，并且光化学稳定性高，不易分解等诸多优点。