EtoposideDescription
TopoGENprovidesthisreagentasacontrolpoison/inhibitoroftopoisomeraseII. Forinvitrocleavagedetection,werecommendthatthedrugbetestedoverawiderangeofuMconcentrations;however,cleavagesarestronglyinfluencedbytheamountoftopoisomeraseinthereactionaswellasthetypeofassaybeingemployed(SDSK+vs.RADIoactiveEndLabeledDNAvs.testingforplasmidcleavages). Notethatlargeamountsofenzymewillberequiredtodetectcleavagecomplex,comparedtoassaysthatmeasurecatalyticactivity.

ChemicalName: 9-[(4,6-O-Ethylidene-β-D-glucopyranosyl)oxy]-5,8,-8a,9-tetrahydro-5-(4-hydroxy-3,5-dimethoxyphenyl)-furo[3',4':6,7]naphtho[2,3-d]-1,3-dioxol-6(5aH)-one

MolecularFormula:C29H32O13

MolecularWeight:588.56
 

VialContents:
TG4140containslyophilizedEtoposide.Additionof0.25mlofsolvent(DMSO)todrugwillyieldaworkingstockof10mM. Thedrugispre-testedandshowntoinducetopocleavagesusingpurifiedhumantopoisomerasefromTopoGEN.
 

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