Doxorubicin(Adriacin)Description
TopoGENprovidesthisreagentasacontrolpoison/inhibitoroftopoisomeraseII. Forinvitrocleavagedetection,werecommendthatthedrugbetestedoverawiderangeofuMconcentrations;however,cleavagesarestronglyinfluencedbytheamountoftopoisomeraseinthereactionaswellasthetypeofassaybeingemployed(SDSK+vs.RADIoactiveEndLabeledDNAvs.testingforplasmidcleavages). Notethatlargeamountsofenzymewillberequiredtodetectcleavagecomplex,comparedtoassaysthatmeasurecatalyticactivity.

MolecularName: (8S-cis)10-[(3-Amino-2,3,6-trideoxy-α-L-lyxo-hexopyranosyl)oxy-7,8,9,10-tetrahydro-6,8,11-trihydroxy-8-(hydroacetyl)-1-methoxy-5,12-naphthacenedione

MolecularFormula:C27H29NO11.HCl

MolecularWeight:579.99

VialContents:
TG4120issuppliedalreadysUSPendedinwaterat10mM(0.25ml)andisreadyforuse. Thedrugispre-testedandshowntoinducetopocleavagesusingpurifiedhumantopoisomerasefromTopoGEN.

 

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