EllipticineDescription
EllipticineisatopoisomeraseIIpoisonthatinducestopoIIdependentcleavages. 

ChemicalName:5,11-Dimethyl-6H-Pyrido[4,3]Carbazole

MolecularFormula:C17H14N2

MolecularWeight:246.31

TopoGENprovidesthisreagentasacontrolpoison/inhibitoroftopoisomeraseII. Forinvitrocleavagedetection,werecommendthatthedrugbetestedoverawiderangeofuMconcentrations;however,cleavagesarestronglyinfluencedbytheamountoftopoisomeraseinthereactionaswellasthetypeofassaybeingemployed(SDSK+vs.RADIoactiveEndLabeledDNAvs.testingforplasmidcleavages). Notethatlargeamountsofenzymewillberequiredtodetectcleavagecomplex,comparedtoassaysthatmeasurecatalyticactivity.

VialContents:
TG4170issuppliedalreadysUSPendedinDMSOat10mM(0.25ml)andisreadyforuse. Thedrugispre-testedandshowntoinducetopocleavagesusingpurifiedhumantopoisomerasefromTopoGEN.
 

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