Theimmunoprecpitation(IP)kitcontainsthekeyreagentsandprotocolforimmunoprecipitatingp170humantopoisomeraseII. Themethodshouldworkwellwithothercellsbesideshumans. Forexample,mouse,ratandmonkeycellswillcrossreactwithtopoisomeraseIIantibody. Theantibodyisspecificforp170(topoIIalpha)inhuman(HeLa)cellsandalthoughwepresumeitwillbehavesimilarlyinotherspecieswithcrossreactivetopoIIisozymes,thisshouldbecarefullyevaluated.

KitContents:

• Anti-topoII(p170)Antibody,(TG2011-1).Thisisarabbitantibody(polyclonal)directedagainsttheC-Terminalregionofp170.Apeptidewasusedtoraisethisantiserum;theantibodyhasbeenimmunoaffinitypurified.TheantibodyisspecificfortopoIIalpha(p170)form.Kitcontains100ulofantibody.
• HumantopoIIp170Marker.Themarkerisreadytoloadin1XSDSPAGELoADIngbuffer.Loading8-10ulofthismarkerwillgiveareadilydetectablebandoncommassiebluestaining.Kitcontains100ulofmarker.
• DetailedProtocolforimmunoprecipitation
• DetailedProtocolforpreparationofnuclearextracts

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