PolyclonalAntibodytoHumanTopoisomeraseII(p170)-idealforWesternblotting,immunofluorescenceandimmunoprecipitation
Apolyclonalantibodywaspreparedagainsta16residueoligopeptidederivedfromthecarboxyterminalregionofhumantopoisomeraseII.Thepurityofthepeptidewasgreaterthan95%asdeterminedbyHPLCandmassspectralanalysis.TheantibodytiterwasdeterminedbyELISAtobe>1:104.ImmunoaffinitygelchromatographyandantibodypurificationstepswerecarriedouttoobtainahomogeneouslypurifiedIgG.

ThisantibodyisidealforeitherWesternblottingapplicationsor immunoflourecence(IF)studiesonthecytologicallocalizationoftopoisomeraseII.Thedilutionsofantibodyrequiredforspecificdetectionshouldbeoptimizedfortheinvestigator'sparticularsystem(celllinebeingusedforexample);however,wesupplytheantibodyreagentalongwithadetailedprotocolforimmunofluorescenceusingatypicalhumancellline(optimizedforHeLacells).Asecondreagent,the16-meroligopeptide(TG2011-2)isalsoavailable.ThisallowstheinvestigatortoperformpropercontrolsforabsorbingtheantibodytodemonstratespecificityinIFexperiments.TheIFprotocolhasbeenoptimizedfortumorcellsthatpossessrelativelyhighlevelsoftopoisomeraseII.Inaddition,weofferaWesternblotMarkersetfortopoII(TG2011-3)tounambiguouslylocatethepositionofthe170kDatopoII.

PolyclonalAntibodyUnitDefinition:
TheantibodyconcentrationisinWesternblottingunits;oneunitcorrespondstoa1:1000dilutionoftheantibodyrequiredtomake1mlofdilutedprobeforaroutineWesternblot.Thus,10unitsofantibodywillmake10mlofdilutedprobe.

Materialsafetydata:
THISPRODUCEISNOTLICENSEDORAPPROVEDFORADMINISTRATIONTOHUMANSORTOANIMALSOTHERTHANEXPERIMENTALANIMALS.

Theproductissoldasanantibodyforresearchpurposesonly.Safelabpracticesshouldbeemployedwhenhandlingthisproduct.Chemical,physicalandtoxicologicalanalysesoftheproducthavenotbeenperformed.
 

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