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商品描述
Overview:
ProductName | TKDPeptide(Hsp70Peptide):FITC |
Description | Hsp70Peptide |
Purity | >98% |
MolecularFormula | FITC-Abu-TKDNNLLGRFELSG |
MolecularWeight | 1363 |
Properties
StorageTemperature | -20ºC |
ShippingTemperature | ShippedAmbient |
ProductType | Activator |
Solubility | Inaqueoussolution |
Source | Synthetic |
Appearance | Solid |
SafetyPhrases | Classification:Caution:Substancenotyetfullytested.SafetyPhrases:S22-DonotbreathedustS24/25-AvoidcontactwithskinandeyesS36/37/39-Wearsuitableprotectiveclothing,glovesandeye/faceprotection |
CiteThisProduct | TKDPeptide(Hsp70Peptide):FITC(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SIH-119) |
BIOLOGicalDescription
ResearchAreas | Cancer,HeatShock |
ScientificBackground | Hsp70genesencodeabundantheat-inducIBLe70-kDahsps(hsp70s).Inmosteukaryoteshsp70genesexistaspartofamultigenefamily.Theyarefoundinmostcellularcompartmentsofeukaryotesincludingnuclei,mitochondria,chloroplasts,theendoplasmicreticulumandthecytosol,aswellasinbacteria.Thegenesshowahighdegreeofconservation,havingatleast5O%identity(1).TheN-terminaltwothirdsofhsp70saremoreconservedthantheC-terminalthird.Hsp70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides(2).Whenhsc70(constitutivelyexpressed)presentinmammaliancellswastruncated,ATPbindingactivitywasfoundtoresideinanN-terminalfragmentof44kDawhichlackedpeptidebindingcapacity.PolypeptidebindingABIlitythereforeresidedwithintheCterminalhalf(3).ThestructureofthisATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins(4).Heatshockprotein70hasalsobeenfoundtohaveahighdegreeofplasmamembranelocalizationintumourcelllines.Thisiscorrelatedwithincreasedsensitivitytolysismediatedbynaturalkiller(NK)cells.TheTKDpeptideisapartialhsp70sequence(TKDNNLLGRFELSG-aa.450-463)andhasidenticaleffectsonNKcellsasfull-lengthHsp70protein,beingabletostimulatenaturalkillercellsactivityatequivalentconcentrationstofull-lengthHsp70protein(5).IncubationofperipheralbloodlymphocytecellswithTKDpluslow-doseinterleukin2(IL-2)enhancesthecytolyticactivityofNKcellsagainstHsp70membrane-positivetumors,invitroandinvivo(6).LookingformoreinformationonHSP70?VisitournewHSP70ScientificResourceGuideathttp://www.HSP70.com. |
References | 1.BoorsteinW.R.,ZiegelhofferT.&CraigE.A.(1993)J.Mol.Evol.38(1):1-17. 2.RothmanJ.(1989),Cell59:591-601. 3.DeLuca-Flahertyetal.(1990)Cell62:875-887. 4.BorkP.,SanderC.&ValenciaA.(1992)Proc.NatlAcad.Sci.USA89:7290-7294. 5.MulthoffG.,etal.(2001)CellStressChaperones6(4):337-344. 6.Krause,S.W.etal.(2004)Clin.CancerRes.10(11):3699-3707. |
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