AntibodyDilution WB(1:1000),ICC/IF(1:100);optimaldilutionsforassaysshouldbedeterminedbytheuser. Conjugates AlkalinePhosphatase,APC,ATTO390,ATTO488,ATTO565,ATTO594,ATTO633,ATTO655,ATTO680,ATTO700,Biotin,FITC,HRP,PE/ATTO594,PerCP,RPE,Streptavidin,Unconjugated PerCP Overview: Peridinin-Chlorophyll-ProteinComplex Smallphycobiliprotein Isolatedfromredalgae Largestokesshift(195nm) MolecularWeight:35kDa PerCPDatasheet
OpticalProperties: λex =482nm
λem =677nm
εmax =1.96x106
Laser =488nm
PE/ATTO594 PE/ATTO594isatandemconjugate,wherePEisexcitedat535nmandtransfersenergytoATTO594viaFRET(fluorescenceresonanceenergytransfer),whichemitsat627nm. Overview: Highfluorescenceyield HighphotostABI Veryhydrophilic Excellentsolubilityinwater Verylittleaggregation PE/ATTO594Datasheet
OpticalProperties: λex =535nm
λem =627 nm
Laser = 488to561nm
FITC(Fluorescein) Overview: Excellentfluorescencequantumyield Highrateofphotobleaching Goodsolubilityinwater Broademissionspectrum pHdependentspectra Molecularformula:C20 H12 O5 Molarmass:332.3g/mol FITC-Fluorescent-conjugate
OpticalProperties: λex =494 nm
λem =520 nm
εmax =7.3×104
Φf = 0.92
τfl =5.0ns
Brightness = 67.2
Laser = 488 nm
Filterset = FITC
ATTO700 Overview: Highfluorescenceyield Excellentthermalandphotostability Quenchedbyelectrondonors Veryhydrophilic Goodsolubilityinpolarsolvents Zwitterionicdye MolarMass:575g/mol ATTO700Datasheet
OpticalProperties: λex =700 nm
λem =719nm
εmax =1.25×105
Φf = 0.25
τfl =1.6ns
Brightness = 31.3
Laser =676nm
Filterset =Cy®5.5
ATTO680 Overview: Highfluorescenceyield Excellentthermalandphotostability Quenchedbyelectrondonors Veryhydrophilic Goodsolubilityinpolarsolvents Zwitterionicdye MolarMass:631g/mol ATTO680Datasheet
OpticalProperties: λex =680nm
λem =700 nm
εmax =1.25×105
Φf = 0.30
τfl =1.7ns
Brightness =37.5
Laser =633 –676nm
Filterset =Cy®5.5
ATTO655 Overview: Highfluorescenceyield Highthermalandphotostability Excellentozoneresistance Quenchedbyelectrondonors Veryhydrophilic Goodsolubilityinpolarsolvents Zwitterionicdye MolarMass:634g/mol ATTO655Datasheet
OpticalProperties: λex =663nm
λem =684nm
εmax =1.25×105
Φf = 0.30
τfl =1.8ns
Brightness = 37.5
Laser =633 –647nm
Filterset =Cy®5
ATTO633 Overview: Highfluorescenceyield Highthermalandphotostability Moderatelyhydrophilic Goodsolubilityinpolarsolvents StableatpH4–11 Cationicdye,perchloratesalt MolarMass:652.2g/mol ATTO633Datasheet
OpticalProperties: λex =629nm
λem =657nm
εmax =1.3×105
Φf = 0.64
τfl =3.2ns
Brightness =83.2
Laser =633 nm
Filterset =Cy®5
ATTO594 Overview: Highfluorescenceyield Highphotostability Veryhydrophilic Excellentsolubilityinwater Verylittleaggregation Newdyewithnetchargeof-1 MolarMass:1137g/mol ATTO594Datasheet
OpticalProperties: λex =601 nm
λem =627nm
εmax =1.2×105
Φf = 0.85
τfl =3.5ns
Brightness =102
Laser =594nm
Filterset =TexasRed®
ATTO565 Overview: Highfluorescenceyield Highthermalandphotostability Goodsolubilityinpolarsolvents Excellentsolubilityinwater Verylittleaggregation Rhodaminedyederivative MolarMass:611g/mol ATTO565Datasheet
OpticalProperties: λex =563nm
λem =592nm
εmax =1.2×105
Φf = 0.9
τfl =3.4n
Brightness =10
Laser =532nm
Filterset = TRITC
ATTO488 Overview: Highfluorescenceyield Highphotostability Veryhydrophilic Excellentsolubilityinwater Verylittleaggregation Newdyewithnetchargeof-1 MolarMass:804g/mol ATTO488Datasheet
OpticalProperties: λex =501nm
λem =523nm
εmax =9.0×104
Φf = 0.80
τfl =4.1ns
Brightness = 72
Laser = 488 nm
Filterset = FITC
ATTO390 Overview: Highfluorescenceyield LargeStokes-shift(89nm) Goodphotostability Moderatelyhydrophilic Goodsolubilityinpolarsolvents Coumarinderivate,uncharged Lowmolarmass:343.42g/mol ATTO390Datasheet
OpticalProperties: λex =390nm
λem =479nm
εmax =2.4×104
Φf = 0.90
τfl =5.0ns
Brightness = 21.6
Laser =365or405nm
APC (Allophycocyanin)Overview: Highquantumyield Largephycobiliprotein 6chromophorespermolecule Isolatedfromredalgae MolecularWeight:105kDa APCDatasheet
OpticalProperties: λex =650nm
λem =660nm
εmax =7.0×105
Φf = 0.68
Brightness = 476
Laser =594or633 nm
Filterset =Cy®5
Streptavidin Properties:
Homo-tetramericproteinpurifiedfromStreptomycesavidinii whichbindsfourbiotinmoleculeswithextremelyhighaffinity Molecularweight:53kDa Formula:C10 H16 N2 O3 S Applications:Westernblot,immunohistochemistry,andELISA StreptavidinDatasheet
Biotin Properties:
BindstetramericavidinproteinsincludingStreptavidinandneuravidinwithveryhighaffinity Molarmass:244.31g/mol Formula:C10 H16 N2 O3 S Applications:Westernblot,immunohistochemistry,andELISA BiotinDatasheet
HRP(HorserADI Properties:
Enzymaticactivityisusedtoamplifyweaksignalsandincreasevisibilityofatarget Readilycombineswithhydrogenperoxide(H2 O2 )toformHRP-H2 O2 complexwhichcanoxidizevarioushydrogendonors Catalyzestheconversionof:Chromogenicsubstrates(e.g.TMB,DAB,ABTS)intocolouredproducts Chemiluminescentsubstrates(e.g.luminolandisoluminol)intolightemittingproductsviaenhancedchemiluminescence(ECL) Fluorogenicsubstrates(e.g.tyramine,homovanillicacid,and4-hydroxyphenylaceticacid)intofluorescentproducts Highturnoverrateenablesrapidgenerationofastrongsignal 44kDaglycoprotein Extinctioncoefficient:100(403nm) Applications:Westernblot,immunohistochemistry,andELISA HRPDatasheet
AP(AlkalinePhosphatase) Properties:
Broadenzymaticactivityforphosphateestersofalcohols,amines,pyrophosphate,andphenols Commonlyusedtodephosphorylatethe5’-terminiofDNAandRNAtopreventself-ligation Catalyzestheconversionof:Chromogenicsubstrates(e.g.pNPP,naphtholAS-TRphosphate,BCIP)intocolouredproducts Fluorogenicsubstrates(e.g.4-methylumbelliferylphosphate)intofluorescentproducts Molecularweight:140kDa Applications:Westernblot,immunohistochemistry,andELISA APDatasheet
R-PE (R-Phycoerythrin)Overview: Broadexcitationspectrum Highquantumyield Photostable Memberofthephycobiliproteinfamily Isolatedfromredalgae Excellentsolubilityinwater MolecularWeight:250kDa R-PEDatasheet
OpticalProperties: λex =565nm
λem =575nm
εmax =2.0×106
Φf = 0.84
Brightness =1.68x103
Laser = 488to561nm
Filterset = TRITC
ProductImages Immunocytochemistry/ImmunofluorescenceanalysisusingMouseAnti-TASK1PotassiumChannelMonoclonalAntibody,CloneS374-48(SMC-473).Tissue:NeuroblastomacelllineSK-N-BE.Species:Human.Fixation:4%Formaldehydefor15minatRT.PrimaryAntibody:MouseAnti-TASK1PotassiumChannelMonoclonalAntibody(SMC-473)at1:100for60minatRT.SecondaryAntibody:GoatAnti-MouseATTO488at1:100for60minatRT.Counterstain:PhalloidinTexasRedF-Actinstain;DAPI(blue)nuclearstainat1:1000,1:5000for60minRT,5minRT.Localization:Membrane.Magnification:60X.(A)DAPI(blue)nuclearstain(B)PhalloidinTexasRedF-Actinstain(C)TASK1PotassiumChannelAntibody(D)Composite.
WesternBlotanalysisofRatBrainMembraneshowingdetectionof~50kDaTASK1PotassiumChannelproteinusingMouseAnti-TASK1PotassiumChannelMonoclonalAntibody,CloneS374-48(SMC-473).Lane1:MolecularWeightLadder(MW).Lane2:Ratbrainmembrane.Load:15µg.Block:2%BSAand2%SkimMilkin1XTBST.PrimaryAntibody:MouseAnti-TASK1PotassiumChannelMonoclonalAntibody(SMC-473)at1:1000for16hoursat4°C.SecondaryAntibody:GoatAnti-MouseIgG:HRPat1:2000for60minatRT.ColorDevelopment:ECLsolutionfor6minatRT.Predicted/ObservedSize:~50kDa.
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ATTO565 Overview: Highfluorescenceyield Highthermalandphotostability Goodsolubilityinpolarsolvents Excellentsolubilityinwater Verylittleaggregation Rhodaminedyederivative MolarMass:611g/mol ATTO565Datasheet
OpticalProperties: λex =563nm
λem =592nm
εmax =1.2×105
Φf = 0.9
τfl =3.4n
Brightness =10
Laser =532nm
Filterset = TRITC
StressMarq细胞信号生物体中的每个细胞都暴露于来自其环境的数百种不同信号。单元可以以多种方式响应这些信号，并且独立地响应。但是，所有这些独立的动作在控制基本细胞活动的非常复杂且相互联系的通信路径中融合在一起。细胞外信号通过各种信号网络传输信息，最终在细胞核水平发挥作用。小区信令或蜂窝通信的三个阶段包括：接收信号信号转导单元内的响应 倾向于起源于质膜水平的信号传导途径或网络包括G蛋白偶联受体，（非受体）酪氨酸激酶和离子调节通道等。尽管这些过程中涉及的关键调节分子有所不同，但信号的传输通常涉及一个共同的过程。靶蛋白的可逆磷酸化。两种不同的酶介导了这一点：蛋白激酶–磷酸化蛋白质（添加磷酸基团）蛋白质磷酸酶–使蛋白质脱磷酸（去除磷酸基团）细胞感知并正确响应其微环境的能力是发育，组织修复，免疫以及正常组织动态平衡的基础。细胞内信号传导事件实际上调节了所有细胞活动。细胞信息处理中的错误可能导致癌症，自身免疫性疾病和糖尿病等疾病。通过了解细胞信号传导的基本原理，研究人员希望能够开发出新药并对其进行选择性和有效治疗。细胞信号研究需要大量的生命科学产品。我们致力于开发最先进的研究产品，以协助研究细胞信号传导，包括单克隆抗体，多克隆抗体，抗体结合物，蛋白质，免疫测定法和小分子抑制剂。
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