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StressMarq/StressXpress® Retinol Binding Protein Urinary EIA Kit/SKT-218-96/96-well

价格
¥9140.00
货号:SKT-218-96
浏览量:127
品牌:StressMarq
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商品描述

Overview:

ProductNameRetinolBindingProteinUrinaryEIAKit
Description

ColorimetricmeasurementofRBPlevelsinurine

SpeciesReactivityDog,Human,Monkey,Rat
PlatformMicroplate
SampleTypesUrine
DetectionMethodColorimetricAssay
AssayTypeSandwichEIA(EnzymeImmunoassay)
UtilityEIAkitusedtomeasureRBPlevelsinurinesamples.
Sensitivity2.90ng/ml
AssayRange3.906-1000ng/ml
PrecisionIntraAssayPrecision:Fourhumanurinesampleswerediluted1:2withAssayBufferandruninreplicatesof8inanassay.ThemeanandprecisionofthecalculatedRBPconcentrationswere:Sample1-14.8ng/mL,4.5%CVSample2-28.0ng/mL,7.5%CVSample3-53.5ng/mL,7.3%CVSample4-323.7ng/mL,2.1%CVInterAssayPrecision:Fourhumanurinesampleswerediluted1:2withAssayBufferandruninduplicatesintwenty-oneassaysrunovermultipledaysbythreeoperators.ThemeanandprecisionofthecalculatedRBPconcentrationswere:Sample1-14.4ng/mL,9.1%CVSample2-28.0ng/mL,9.2%CVSample3-50.8ng/mL,8.1%CVSample4-309.2ng/mL,14.0%CV
IncubationTime90Minutes
NumberofSamples41samplesinduplicate
OtherResourcesKitBooklet,MSDS

Properties

StorageTemperature4ºC
ShippingTemperatureBlueIce
ProductTypeEIAKits
AssayOverviewTheRetinolBindingProteinUrinaryEIAKitisdesignedtoquantitativelymeasureRBPpresentinurinesamples.ARBPstandardisprovidedtogenerateastandardcurvefortheassayandallsamplesshouldbereadoffthestandardcurve.StandardsordilutedsamplesarePipettedintoaclearmicrotiterplatecoatedwithanantibodytocapturerabbitantibodies.ARBP-peroxidaseconjugateisaddedtothestandardsandsamplesinthewells.ThebindingreactionisinitiatedbytheadditionoftheRBPpolyclonalantibodytoeachwell.Afteranhourincubationtheplateiswashedandsubstrateisadded.ThesubstratereactswiththeboundRBP-peroxidaseconjugate.Afterashortincubation,thereactionisstoppedandtheintensityofthegeneratedcolorisdetectedinamicrotiterplatereadercapableofmeasuring450nmwavelength.TheconcentrationoftheRBPinthesampleiscalculated,aftermakingasuitablecorrectionforthedilutionofthesample,usingsoftwareavailablewithmostplatereaders.
KitOverview
ComponentNo.ItemQuantity/Size
SKC-218AClear96wellPlate1Plate
SKC-218BRBPStandard60µl
SKC-218CStressXpress®RBPAntibody3ml
SKC-218DStressXpress®RBP-PeroxidaseConjugate3ml
SKC-218EAssayBuffer28ml
SKC-218FWashBufferConcentrate30ml
SKC-218GTMBSubstrate11ml
SKC-218HStopSolution11ml
SKC-218IPlateSealer1Each
CiteThisProductRetinolBindingProteinUrinaryEIAKit(StressMarqBiosciencesInc.,VictoriaBCCANADA,Catalog#SKT-218)

BIOLOGicalDescription

AlternativeNamesCellularretinolbindingproteinEIAKit,CRBPEIAKit,CRBPEIAKit,CRBP1EIAKit,CRBP2EIAKit,RBP1EIAKit,RBP2EIAKit,RBP4EIAKit,RBPCEIAKit,Retinolbindingprotein1EIAKit,Retinolbindingprotein2EIAKit,Retinolbindingprotein4EIAKit
ResearchAreasCardiovascularSystem,CellSignaling
ScientificBackgroundRetinolbindingprotein(RBP)isfromafamilyofstructurallyrelatedproteinsthatbindsmallhydrophobicmoleculessuchasbilepigments,steroids,odorants,etc(1).RBPisa21kDahighlyconserved,single-chainglycoprotein,consistingof182aminoacidswith3disulfidebonds,thathasahydrophobicpocketwhichbindsretinol(vitaminA).RBPbindsretinolina1:1stoichiometry,whichservestonotonlysolubilizeretinolbutalsoprotectitfromoxidation.Wheninserum,themajorityofRBPboundwithretinolisreversIBLycomplexedwithtransthyretin(prealbumin)(2,3).Thiscomplexthentransportsretinoltospecificreceptorsofvarioustissuesinthebody.VitaminAstatusisreflectedbyserumconcentrationasitishemostaticallycontrolledanddoesnotfalluntilstoresaredramaticallyreduced(4,5).RBPhasalsobeenshowntobeausefulMarkerforrenalfunction(6)asitistotallyfilteredbytheglomeruliandreabsorbedbyproximaltubules(7).ThishasmadeurinaryRBP(uRBP)atooltostudyrenalfunctioninheart(8)orkidney(9)transplantrecipients,type1and2diabetics(10),andinpeopleexposedtouraniumfromminingoperations(11).MeasurementofuRBPlevelshasalsobeenusefulindetectionandcharacterizationofdiseasesincludinghypertension(12)andcertaincancers(13,14).
References1.BlanerWS.EndocrRev.1989Aug;10(3):308–16.
2.WolfG.Physiol.Rev.1984Jul;64(3):873-937.
3.PetersenPA.J.Biol.Chem.1971,246:34-43.
4.GoodmanDS,BlanerWS.inTheRetinoids,eds.SpornMB,RobertsAB,GoodmanDS,(Orlando:AcademicPress,1984)vol.2,1-39.
5.Olson,JA.ModernNutritioninHealthandDisease,eds.ShilsME,OlsonJA,ShikeM,(Philadelphia:Lea&Febiger,1994)8thed.287–307.
6.PetersonPA,andBerggårdI.J.Biol.Chem.1971246:25-33.
7.BernardAM,andLauwerysRR.Clin.Chem.198127:1781-2.
8.CamaraNO,MatosAC,RodriguesDAetal.TheLancet2001357:856-857.
9.HosakaB,ParkSI,FelipeCR,GarciaRG,MachadoPG,PereiraAB,Tedesco-SilvaHandMedina-PestanaJO.TransplantProc.200335:1341-1343.
10.AbahusainMA,WrightJ,DickersonJWTanddeVolEB.AmJClinNutr.2004;79:218-25.
11.WyattSA,ReitzLV,CroleyTR,HawkinsD,BarrettE,McKeownA,PowellN,WestA,HamnerTandRoysterMO.JExpSciEnvironEpidem.200818:59-75.
12.BangLE,HolmJandSvendsenTL.Am.J.Hypertens19969:1024-1028.
13.MoiseAR,NoyN,PalczewskiK,BlanerWS.Biochemistry20079:1024-1028.
14.GavrilovV,YermiahuT,GorodischerR.KidneyInt.2006Jan;69(1):173-7.

ProductImages

<p>Typical Standard Curve for the Retinol Binding Protein Urinary EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-218. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 3.906 – 1000 ng/ml.</p>

TypicalStandardCurvefortheRetinolBindingProteinUrinaryEIAKit(EnzymeImmunoassay)StressXpress®–SKT-218.AssayType:SandwichEIA.DetectionMethod:ColorimetricAssay.AssayRange:3.906–1000ng/ml.

<p>Linearity was determined by taking two human urine samples diluted 1:2, one with a low diluted RBP level of 14.5 ng/mL and one with a higher diluted level of 299.2 ng/mL and mixing them in given ratios. The measured concentrations were compared to the expected values based on the ratios used.</p>

Linearitywasdeterminedbytakingtwohumanurinesamplesdiluted1:2,onewithalowdilutedRBPlevelof14.5ng/mLandonewithahigherdilutedlevelof299.2ng/mLandmixingthemingivenratios.Themeasuredconcentrationswerecomparedtotheexpectedvaluesbasedontheratiosused.

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